Overview
- Editors:
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Sam Massa
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Cellular and Molecular Immunology Laboratory, Vrije Universiteit Brussel, Brussels, Belgium
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Nick Devoogdt
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In Vivo Cellular and Molecular Imaging Laboratory (ICMI), Vrije Universiteit Brussel, Brussels, Belgium
- Includes cutting-edge techniques
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
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Table of contents (20 protocols)
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- Stephan Dickgiesser, Roland Kellner, Harald Kolmar, Nicolas Rasche
Pages 1-14
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- MaurÃcio Morais, Nafsika Forte, Vijay Chudasama, James R. Baker
Pages 15-24
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- Maria J. Matos, Gonzalo Jiménez-Osés, Gonçalo J. L. Bernardes
Pages 25-37
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- Alex R. Nanna, Christoph Rader
Pages 39-52
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- Jared L. Spidel, Earl F. Albone
Pages 53-65
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- Rania A. Hashad, Jaclyn L. Lange, Natasha C. W. Tan, Karen Alt, Christoph E. Hagemeyer
Pages 67-80
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- Makan Khoshnejad, Jacob S. Brenner, Hamideh Parhiz, Vladimir R. Muzykantov
Pages 81-93
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- Sandra Liebscher, Frank Bordusa
Pages 95-115
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- Geert-Jan Graulus, Duy Tien Ta, Huong Tran, Rebekka Hansen, Brecht Billen, Erik Royackers et al.
Pages 117-130
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- Junjie Liu, Robyn M. Barfield, David Rabuka
Pages 131-147
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- Karin F. K. Ejendal, Julia G. Fraseur, Tamara L. Kinzer-Ursem
Pages 149-165
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- Dominik Schumacher, Heinrich Leonhardt, Christian P. R. Hackenberger, Jonas Helma
Pages 167-189
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- Christopher R. Drake, Michael J. Evans, Henry F. VanBrocklin
Pages 191-205
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- Yi Zhang, Shelby Auger, Jonas V. Schaefer, Andreas Plückthun, Mark D. Distefano
Pages 207-219
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- Feng Tang, Wei Shi, Wei Huang
Pages 221-238
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- Partha Sarathi Addy, Sarah B. Erickson, James S. Italia, Abhishek Chatterjee
Pages 239-251
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- Hannes Merten, Jonas V. Schaefer, Fabian Brandl, Uwe Zangemeister-Wittke, Andreas Plückthun
Pages 253-273
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- Fabiana Zappala, Andrew Tsourkas
Pages 275-286
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- Xiao-Wei Wang, Wen-Bin Zhang
Pages 287-300
About this book
This book explores well-established and emerging conjugation strategies that are relevant for proteins used in the field of precision medicine, focusing on techniques that are suitable for antibodies, antibody-fragments such as Fabs, scFvs, or nanobodies, scaffold proteins such as FN3 or DARPin, peptides, or model proteins. Although centered on the development of bioconjugates rather than their application, most protocols also show the conjugation of the targeting vehicle to a diagnostic or therapeutic entity, with the end-product most often being an antibody-drug conjugate, an optical probe, a nanomedicine, or a radiopharmaceutical. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Bioconjugation: Methods and Protocols is an ideal guide for researchers looking toward precision medicine in order to expand the vital field of drug discovery.
Editors and Affiliations
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Cellular and Molecular Immunology Laboratory, Vrije Universiteit Brussel, Brussels, Belgium
Sam Massa
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In Vivo Cellular and Molecular Imaging Laboratory (ICMI), Vrije Universiteit Brussel, Brussels, Belgium
Nick Devoogdt