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Enzyme-Mediated Ligation Methods

  • Book
  • © 2019

Overview

Editors:
  1. Timo Nuijens

    1. EnzyPep B.V., Geleen, The Netherlands

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    You can also search for this editor in PubMed   Google Scholar

  2. Marcel Schmidt

    1. EnzyPep B.V., Geleen, The Netherlands

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2012)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Sortase A Enzyme-Mediated Generation of Site-Specifically Conjugated Antibody–Drug Conjugates

    • Rémy Gébleux, Manfred Briendl, Ulf Grawunder, Roger R. Beerli
    Pages 1-13

  3. Site-Specific C-Terminal Labeling of Recombinant Proteins with Proximity-Based Sortase-Mediated Ligation (PBSL)

    • Hejia Henry Wang, Andrew Tsourkas
    Pages 15-28

  4. Cyclizing Disulfide-Rich Peptides Using Sortase A

    • Akello J. Agwa, David J. Craik, Christina I. Schroeder
    Pages 29-41

  5. Chemoenzymatic Synthesis of Linear- and Head-to-Tail Cyclic Peptides Using Omniligase-1

    • Marcel Schmidt, Timo Nuijens
    Pages 43-61

  6. Site-Specific Labeling of Proteins Using the Formylglycine-Generating Enzyme (FGE)

    • Igor Rupniewski, David Rabuka
    Pages 63-81

  7. Butelase 1-Mediated Ligation of Peptides and Proteins

    • Xinya Hemu, Xiaohong Zhang, Xiaobao Bi, Chuan-Fa Liu, James P. Tam
    Pages 83-109

  8. Trypsiligase-Catalyzed Peptide and Protein Ligation

    • Sandra Liebscher, Frank Bordusa
    Pages 111-133

  9. Site-Specific Antibody–Drug Conjugation Using Microbial Transglutaminase

    • Stephan Dickgiesser, Lukas Deweid, Roland Kellner, Harald Kolmar, Nicolas Rasche
    Pages 135-149

  10. Tailoring Activity and Selectivity of Microbial Transglutaminase

    • Lukas Deweid, Olga Avrutina, Harald Kolmar
    Pages 151-169

  11. SpyLigase-Catalyzed Modification of Antibodies

    • Vanessa Siegmund, Birgit Piater, Frank Fischer, Harald Kolmar
    Pages 171-192

  12. Peptide Cyclization Catalyzed by Cyanobactin Macrocyclases

    • Wael E. Houssen
    Pages 193-210

  13. In Vitro and In Planta Cyclization of Target Peptides Using an Asparaginyl Endopeptidase from Oldenlandia affinis

    • Karen S. Harris, Simon Poon, Pedro Quimbar, Marilyn A. Anderson
    Pages 211-235

  14. Site-Specific Antibody Labeling Using Phosphopantetheinyl Transferase-Catalyzed Ligation

    • Jan Grünewald, Ansgar Brock, Bernhard H. Geierstanger
    Pages 237-278

  15. Lipoic Acid Ligase-Promoted Bioorthogonal Protein Modification and Immobilization

    • Joseph G. Plaks, Joel L. Kaar
    Pages 279-297

  16. BioID as a Tool for Protein-Proximity Labeling in Living Cells

    • Rhiannon M. Sears, Danielle G. May, Kyle J. Roux
    Pages 299-313

  17. N-Myristoyl Transferase (NMT)-Catalyzed Labeling of Bacterial Proteins for Imaging in Fixed and Live Cells

    • Samuel H. Ho, David A. Tirrell
    Pages 315-326

  18. Tubulin Tyrosine Ligase-Mediated Modification of Proteins

    • Marcus Gerlach, Tina Stoschek, Heinrich Leonhardt, Christian P. R. Hackenberger, Dominik Schumacher, Jonas Helma
    Pages 327-355

  19. Inducible, Selective Labeling of Proteins via Enzymatic Oxidation of Tyrosine

    • Jorick J. Bruins, Criss van de Wouw, Jordi F. Keijzer, Bauke Albada, Floris L. van Delft
    Pages 357-368

  20. Back Matter

    Pages 369-371
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Keywords

About this book

This volume discusses different enzyme-catalyzed ligation methodologies for a variety of different chemical transformations. This book wants readers to view enzymes as a powerful tool in both academic and industrial research. Chapters in this book cover topics such as sortase A-mediated generation of site-specifically conjugated antibody-drug conjugates; omniligase-catalyzed inter- and intramolecular ligation; ligation catalyzed by microbial transglutaminase; peptide cyclization mediated by cyanobactin macrocyclases, butelase 1 and sortase A; using BioID as a tool for protein proximity labeling in living cells; and inducible, selective labeling of proteins via enzymatic oxidation of tyrosine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Enzyme-Mediated Ligation Methods is a valuable resource for students and scientists from different disciplines who are interested in using enzymatic strategies to answer their research questions. 



Editors and Affiliations

  • EnzyPep B.V., Geleen, The Netherlands

    Timo Nuijens, Marcel Schmidt

Bibliographic Information

  • Book Title: Enzyme-Mediated Ligation Methods

  • Editors: Timo Nuijens, Marcel Schmidt

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9546-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9545-5Published: 03 June 2019

  • Softcover ISBN: 978-1-4939-9548-6Published: 14 August 2020

  • eBook ISBN: 978-1-4939-9546-2Published: 03 June 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 371

  • Number of Illustrations: 35 b/w illustrations, 59 illustrations in colour

  • Topics: Biotechnology, Protein-Ligand Interactions, Enzymology

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