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Lipid-Protein Interactions

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Jörg H. Kleinschmidt

    1. Department of Biophysics, Institute of Biology, FB10 and Center for Interdisciplinary Nanostructure Science and Technology (CINSaT), University of Kassel, Kassel, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2003)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Multiscale Modeling and Simulation Approaches to Lipid–Protein Interactions

    • Roland G. Huber, Timothy S. Carpenter, Namita Dube, Daniel A. Holdbrook, Helgi I. Ingólfsson, William A. Irvine et al.
    Pages 1-30

  3. Quartz Crystal Microbalances as Tools for Probing Protein–Membrane Interactions

    • Søren B. Nielsen, Daniel E. Otzen
    Pages 31-52

  4. Surface Plasmon Resonance for Measuring Interactions of Proteins with Lipids and Lipid Membranes

    • Aleksandra Šakanovič, Vesna Hodnik, Gregor Anderluh
    Pages 53-70

  5. Thermodynamic Analysis of Protein–Lipid Interactions by Isothermal Titration Calorimetry

    • Musti J. Swamy, Rajeshwer S. Sankhala, Bhanu Pratap Singh
    Pages 71-89

  6. Differential Scanning Calorimetry of Protein–Lipid Interactions

    • Olga Cañadas, Cristina Casals
    Pages 91-106

  7. Imaging and Force Spectroscopy of Single Transmembrane Proteins with the Atomic Force Microscope

    • K. Tanuj Sapra
    Pages 107-144

  8. Kinetics of Insertion and Folding of Outer Membrane Proteins by Gel Electrophoresis

    • Andre Schüßler, Sascha Herwig, Jörg H. Kleinschmidt
    Pages 145-162

  9. Optimized Negative-Staining Protocol for Lipid–Protein Interactions Investigated by Electron Microscopy

    • Jianfang Liu, Hao Wu, Changyu Huang, Dongsheng Lei, Meng Zhang, Wei Xie et al.
    Pages 163-173

  10. Probing Heterogeneous Lipid Interactions with Membrane Proteins Using Mass Spectrometry

    • John W. Patrick, Arthur Laganowsky
    Pages 175-190

  11. Protein Microarrays and Liposome: A Method for Studying Lipid–Protein Interactions

    • Samuel Herianto, Chien-Sheng Chen, Heng Zhu
    Pages 191-199

  12. Structural Investigations of Protein–Lipid Complexes Using Neutron Scattering

    • Luke A. Clifton, Stephen C. L. Hall, Najet Mahmoudi, Timothy J. Knowles, Frank Heinrich, Jeremy H. Lakey
    Pages 201-251

  13. Circular-Dichroism and Synchrotron-Radiation Circular-Dichroism Spectroscopy as Tools to Monitor Protein Structure in a Lipid Environment

    • Koichi Matsuo, Kunihiko Gekko
    Pages 253-279

  14. FTIR Analysis of Proteins and Protein–Membrane Interactions

    • Suren A. Tatulian
    Pages 281-325

  15. UV Resonance Raman Spectroscopy as a Tool to Probe Membrane Protein Structure and Dynamics

    • DeeAnn K. Asamoto, Judy E. Kim
    Pages 327-349

  16. Analyzing Transmembrane Protein and Hydrophobic Helix Topography by Dual Fluorescence Quenching

    • Gregory A. Caputo, Erwin London
    Pages 351-368

  17. Förster Resonance Energy Transfer as a Tool for Quantification of Protein–Lipid Selectivity

    • Luís M. S. Loura, Manuel Prieto, Fábio Fernandes
    Pages 369-382

  18. A Guide to Tracking Single Membrane Proteins and Their Interactions in Supported Lipid Bilayers

    • Evan L. Taylor, Kumud Raj Poudel, James A. Brozik
    Pages 383-414

  19. Fluorescence Correlation Spectroscopy to Examine Protein–Lipid Interactions in Membranes

    • Viktoria Betaneli, Jonas Mücksch, Petra Schwille
    Pages 415-447

  20. Membrane Pore Formation by Peptides Studied by Fluorescence Techniques

    • Suren A. Tatulian, Nabin Kandel
    Pages 449-464
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Keywords

About this book

This second edition volume expands all chapters of the previous edition, which have been enhanced to cover the most recent developments, the current state of method research, and applications. Additional protocols were added to examine lipid-protein interactions by mass spectrometry, to use protein microarrays to investigate large sets of various proteins, to study membrane protein dynamics by UV resonance Raman spectroscopy, to analyze peptide-induced pore formation in membranes, and to investigate folding and insertion of membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Lipid-Protein Interactions: Methods and Protocols, Second Edition is an essential resource for all researchers who are interested in obtaining up-to-date and comprehensive information about membrane structure and function.


Editors and Affiliations

  • Department of Biophysics, Institute of Biology, FB10 and Center for Interdisciplinary Nanostructure Science and Technology (CINSaT), University of Kassel, Kassel, Germany

    Jörg H. Kleinschmidt

Bibliographic Information

  • Book Title: Lipid-Protein Interactions

  • Book Subtitle: Methods and Protocols

  • Editors: Jörg H. Kleinschmidt

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9512-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9511-0Published: 25 June 2019

  • Softcover ISBN: 978-1-4939-9514-1Published: 14 August 2020

  • eBook ISBN: 978-1-4939-9512-7Published: 19 June 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIV, 682

  • Number of Illustrations: 55 b/w illustrations, 92 illustrations in colour

  • Topics: Protein Science, Lipidology, Mass Spectrometry

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