Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1999)
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Table of contents (24 protocols)
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Front Matter
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Cellular Assays to Detect and Measure DNA Damage and Damage Response
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Front Matter
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Cellular and Cell Extract-Based Assays to Measure DNA Repair
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Front Matter
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In Vitro Biochemical and Biophysical Methods to Study DNA Repair
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Front Matter
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Keywords
About this book
The volume outlines techniques used to study the primary mechanisms of important DNA repair pathways. The chapters in this book are organized into five sections, each discussing a specific aspect of repair biology. Part I reviews the role of post-translational modifications of HDR, and DNA damage caused by defective telomere replication. Part II describes methods to detect and measure DNA breaks and proteins involved in DNA damage response. Part III talks about methods designed to measure DNA repair efficiency and characterize factors involved in the repair process. Part IV discusses various in vitro assays used to purify recombinant proteins that are used to study DNA repair enzyme mechanisms. Lastly, Part V focuses on methods to study the damage arising during DNA replication. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, DNA Repair: Methods and Protocols is an essential resource for novice and experienced researchers, and provides easily adaptable techniques to help them advance in their specific protein or repair pathway of interest.
Editors and Affiliations
Bibliographic Information
Book Title: DNA Repair
Book Subtitle: Methods and Protocols
Editors: Lata Balakrishnan, Jason A. Stewart
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-9500-4
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019
Hardcover ISBN: 978-1-4939-9499-1Published: 25 May 2019
Softcover ISBN: 978-1-4939-9502-8Published: 14 August 2020
eBook ISBN: 978-1-4939-9500-4Published: 24 May 2019
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XV, 352
Number of Illustrations: 29 b/w illustrations, 64 illustrations in colour
Topics: Human Genetics