Microbial Lipid Production

1. Front Matter

   Pages i-xiv

   PDF

2. Microbial Lipid Alternatives to Plant Lipids

   • A. Daniel Jones, Kyria L. Boundy-Mills, G. Florin Barla, Sandeep Kumar, Bryan Ubanwa, Venkatesh Balan

   Pages 1-32

3. Laboratory Screening Protocol to Identify Novel Oleaginous Yeasts

   • Irnayuli R. Sitepu, Antonio L. Garay, Tomas Cajka , Oliver Fiehn, Kyria L. Boundy-Mills

   Pages 33-50

4. Fungi (Mold)-Based Lipid Production

   • Yan Yang, Fatemeh Heidari, Bo Hu

   Pages 51-89

5. Qualitative and Quantitative Estimation of Bacterial Lipid Production

   • Gangatharan Muralitharan, Manickam Gayathri, Sumathy Shunmugam

   Pages 91-101

6. Rhodococcus and Yarrowia-Based Lipid Production Using Lignin-Containing Industrial Residues

   • Rosemary K. Le, Kristina M. Mahan, Arthur J. Ragauskas

   Pages 103-120

7. Quantification of Lipid Content in Oleaginous Biomass Using Thermogravimetry

   • Balakrishna Maddi, Agasteswar Vadlamani, Sridhar Viamajala, Sasidhar Varanasi

   Pages 121-129

8. Extraction and Characterization of Lipids from Macroalgae

   • David R. Nobles Jr., Schonna R. Manning

   Pages 131-140

9. Genetic Engineering Approaches Used to Increase Lipid Production and Alter Lipid Profile in Microbes

   • Xiao-Ling Tang, Ya-Ping Xue

   Pages 141-150

10. Extraction Methods Used to Separate Lipids from Microbes

    • Balakrishna Maddi

    Pages 151-159

11. Novel Microbial Modification Tools to Convert Lipids into Other Value-Added Products

    • Priya Kumari, Farnaz Yusuf, Naseem A. Gaur

    Pages 161-171

12. Alkaline and Alkaline-Oxidative Pretreatment and Hydrolysis of Herbaceous Biomass for Growth of Oleaginous Microbes

    • Jacob D. Crowe, Muyang Li, Daniel L. Williams, Alex D. Smith, Tongjun Liu, David B. Hodge

    Pages 173-182

13. Laboratory Conversion of Cultivated Oleaginous Organisms into Biocrude for Biofuel Applications

    • Eboibi Blessing, Umakanta Jena, Senthil Chinnasamy

    Pages 183-193

14. Life Cycle Analysis of Producing Microbial Lipids and Biodiesel: Comparison with Plant Lipids

    • Tom Bradley, Daniel Maga

    Pages 195-214

15. Assessment of Fuel Quality Parameters and Selection of Bacteria Using PROMETHEE–GAIA Algorithm

    • Sumathy Shunmugam, Manickam Gayathri, Gangatharan Muralitharan

    Pages 215-227

16. Production of High-Value Polyunsaturated Fatty Acids Using Microbial Cultures

    • Mingjie Jin, Rui Zhai, Zhaoxian Xu, Zhiqiang Wen

    Pages 229-248

17. Screening for Oily Yeasts Able to Convert Hydrolysates from Biomass to Biofuels While Maintaining Industrial Process Relevance

    • Patricia J. Slininger, Bruce S. Dien, Joshua C. Quarterman, Stephanie R. Thompson, Cletus P. Kurtzman

    Pages 249-283

18. Conversion of Microbial Lipids to Biodiesel and Basic Lab Tests for Analysis of Fuel-Quality Parameters

    • Annaliese K. Franz, Cody Yothers

    Pages 285-310

19. Impact of Culture Conditions on Neutral Lipid Production by Oleaginous Yeast

    • Irene Fakankun, Maryam Mirzaei, David B. Levin

    Pages 311-325

20. Producing Oleaginous Microorganisms Using Wastewater: Methods and Guidelines for Lab- and Industrial-Scale Production

    • Kayla M. Rude, Tyler J. Barzee, Annaliese K. Franz

    Pages 327-355

The volume explores methods for isolation and characterization of lipids, and their production using various oleaginous organisms. The protocols presented in the book consider sugars derived from different substrates including chemically pre-treated agricultural residues, industrial residues containing lignin, food wastes, and industrial waste water in an approachable format. The book also discusses applications that use oleaginous organisms to transform substrates into a variety of products including bio-crude, high value fatty acids, biofuels such as biodiesel, neutral lipids, volatile fatty acids, and surfactants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Comprehensive and cutting-edge, Microbial Lipid Production: Methods and Protocols is a valuable resource aimed at novice researchers who are working on improving their knowledge and skills in this developing field. 

• bio-crude
• metabolic pathway
• oleaginous
• substrates
• lipid conversion

• Department of Engineering Technology, Biotechnology Program College of Technology, University of Houston, Houston, USA

  Venkatesh Balan

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