Redox-Mediated Signal Transduction

1. Front Matter

   Pages i-x

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2. The Role of Redox in Signal Transduction

   • John T. Hancock

   Pages 1-11

3. Methods for the Addition of Redox Compounds

   • John T. Hancock

   Pages 13-25

4. Investigating ROS, RNS, and H2S-Sensitive Signaling Proteins

   • Eleanor Williams, Matthew Whiteman, Mark E. Wood, Ian D. Wilson, Michael R. Ladomery, Joel Allainguillaume et al.

   Pages 27-42

5. Measurement of 4-Hydroxynonenal (4-HNE) Protein Adducts by ELISA

   • Kosha Mehta, Vinood B. Patel

   Pages 43-52

6. Using Flow Cytometry to Detect and Measure Intracellular Thiol Redox Status in Viable T Cells from Heterogeneous Populations

   • Alex J. Wadley, Rhys G. Morgan, Richard L. Darley, Paul S. Hole, Steven J. Coles

   Pages 53-70

7. Detection of S-Nitrosation and S-Glutathionylation of the Human Branched-Chain Aminotransferase Proteins

   • Thomas E. Forshaw, Myra E. Conway

   Pages 71-84

8. Imaging of Intracellular Hydrogen Peroxide Production with HyPer upon Stimulation of HeLa Cells with EGF

   • Kseniya N. Markvicheva, Ekaterina A. Bogdanova, Dmitry B. Staroverov, Sergei Lukyanov, Vsevolod V. Belousov

   Pages 85-91

9. Applications of Electron Paramagnetic Resonance (EPR) Spectroscopy in the Study of Oxidative Stress in Biological Systems

   • Simon K. Jackson

   Pages 93-102

10. Extracellular and Intracellular NO Detection in Plants by Diaminofluoresceins

    • Neidiquele Maria Silveira, Eduardo Caruso Machado, Rafael Vasconcelos Ribeiro

    Pages 103-108

11. Working with Hypoxia

    • Elizabeth Bowler, Michael R. Ladomery

    Pages 109-133

12. Predicting the Effects of Low Dose-Rate Ionizing Radiation on Redox Potential in Plant Cells

    • Nicol Caplin, Neil Willey

    Pages 135-142

13. Thioredoxin-1 PEGylation as an In Vitro Method for Drug Target Identification

    • Jolanta Skalska

    Pages 143-149

14. Redox-Regulated, Targeted Affinity Isolation of NADH-Dependent Protein Interactions with the Branched Chain Aminotransferase Proteins

    • Maya E. L. Hindy, Myra E. Conway

    Pages 151-163

15. Analysis of Redox Relationships in the Plant Cell Cycle: Determination of Ascorbate, Glutathione, and Poly(ADPribose)polymerase (PARP) in Plant Cell Cultures

    • Christine H. Foyer, Till K. Pellny, Vittoria Locato, Jonathon Hull, Laura De Gara

    Pages 165-181

16. Equations to Support Redox Experimentation

    • John T. Hancock, Matthew Whiteman

    Pages 183-195

17. Back Matter

    Pages 197-199

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This volume explores the scope of the cellular redox analysis and the importance of not being limited by frequently changing and evolving technology. The chapters in this book cover a wide range of topics such as redox components in animal and plant cells and the role of reactive oxygen species, reactive nitrogen species, and hydrogen sulphide in cell signaling; measuring modifications using Flow Cytometry, ELISA assays, and Western blot analysis; measurement of oxidative stress in mitochondira and biological systems; and the use of the genetically encoded fluorescent probe HyPer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 
Cutting-edge and informative, Redox-Mediated Signal Transduction: Methods and Protocols, Second Edition is a valuable resource for both novice and expert researchers who want to expand their studies into new areas and new systems in the evolving redox field. 

• signaling system
• reactive oxygen species (ROS)
• reactive nitrogen species (RNS)
• hydrogen sulphide in cell signaling
• flow cytometry
• western blot analysis

• Department of Applied Sciences, University of the West of England, Bristol, UK

  John T. Hancock, Myra E. Conway

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