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  • © 2019

Radiation Cytogenetics

Methods and Protocols

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1984)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-x
  2. Animal Lymphocyte Metaphase Chromosome Preparation

    • Kazuki Heishima, Kendon Kuo, Masashi Kimura, Takashi Mori
    Pages 7-22
  3. Micronuclei Formation Analysis After Ionizing Radiation

    • Cathy Su, Alexis H. Haskins, Takamitsu A. Kato
    Pages 23-29
  4. G1 Premature Chromosome Condensation (PCC) Assay

    • Ryuichi Okayasu, Cuihua Liu
    Pages 31-38
  5. Sister Chromatid Exchange as a Genotoxic Stress Marker

    • Shigeaki Sunada, Jeremy S. Haskins, Takamitsu A. Kato
    Pages 61-68
  6. DNA Damage Focus Formation Assay

    • Yoshihiro Fujii
    Pages 69-73
  7. Nuclear Foci Assays in Live Cells

    • Eiichiro Mori, Aroumougame Asaithamby
    Pages 75-85
  8. In Situ DNA Damaging Foci Analysis on Metaphase Chromosomes

    • Chisato Omata, Takamitsu A. Kato
    Pages 87-93
  9. Modified PNA Telomere and Centromere FISH Protocols

    • Ian M. Cartwright
    Pages 101-105
  10. Directional Genomic Hybridization (dGH) for Detection of Intrachromosomal Rearrangements

    • Erin Robinson, Miles J. McKenna, Joel S. Bedford, Edwin H. Goodwin, Michael N. Cornforth, Susan M. Bailey et al.
    Pages 107-116
  11. Reciprocal Translocation Analysis with Whole Chromosome Painting for FISH

    • Jeremy S. Haskins, Takamitsu A. Kato
    Pages 117-122
  12. Back Matter

    Pages 137-138

About this book

This book comprises a collection of chapters describing topics from traditional radiation cytogenetic analysis methods to the modern fluorescence based-analysis and high throughput automatic analysis methods. Cytogenetic analysis is important not only in order to understand radiation-induced DNA damage in cells, but it is also a standard biological method to estimate human radiation exposure in the radiation accidents. Therefore, we prepared a series of chapters not only covering biological aspects but also exploring radiation physics and radiation protection. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and equipment, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Radiation Cytogenetics: Methods and Protocols serves both novices workingon their first experiments as well as experienced researchers searching for tried-and-true techniques to adapt for their own laboratory.

Editors and Affiliations

  • Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, USA

    Takamitsu A. Kato

  • Department of Radiation Oncology, University of California Davis, Sacramento, USA

    Paul F. Wilson

Bibliographic Information

  • Book Title: Radiation Cytogenetics

  • Book Subtitle: Methods and Protocols

  • Editors: Takamitsu A. Kato, Paul F. Wilson

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9432-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9430-4Published: 04 July 2019

  • eBook ISBN: 978-1-4939-9432-8Published: 02 July 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 138

  • Number of Illustrations: 7 b/w illustrations, 11 illustrations in colour

  • Topics: Human Genetics, Cell Culture

Buy it now

Buying options

eBook USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access