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Photosynthesis

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Sarah Covshoff

    1. Niceville, USA

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  • Includes cutting-edge methods and protocols for the study of photosynthesis
  • Provides step-by-step detail essential for the best results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1770)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
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  2. Measuring Photosynthetic Parameters

    1. Front Matter

      Pages 1-1
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    2. Survey of Tools for Measuring In Vivo Photosynthesis

      • Berkley J. Walker, Florian A. Busch, Steven M. Driever, Johannes Kromdijk, Tracy Lawson
      Pages 3-24

    3. Photosynthetic Gas Exchange in Land Plants at the Leaf Level

      • Florian A. Busch
      Pages 25-44

    4. Design and Use of a Digitally Controlled Device for Accurate, Multiplexed Gas Exchange Measurements of the Complete Foliar Parts of Plants

      • Gavin M. George, Katharina Kölling, Roland Kuenzli, Matthias Hirsch-Hoffmann, Patrick Flütsch, Samuel C. Zeeman
      Pages 45-68

    5. Measuring Canopy Gas Exchange Using CAnopy Photosynthesis and Transpiration Systems (CAPTS)

      • Qingfeng Song, Xin-Guang Zhu
      Pages 69-81

    6. Light-Response Curves in Land Plants

      • Robert A. Coe, HsiangChun Lin
      Pages 83-94

    7. Chlorophyll Fluorescence on the Fast Timescale

      • Olubukola O. Ajigboye, Rumiana V. Ray, Erik H. Murchie
      Pages 95-104

    8. Sub-saturating Multiphase Flash Irradiances to Estimate Maximum Fluorescence Yield

      • Thomas J. Avenson, Aaron J. Saathoff
      Pages 105-120

    9. Chlorophyll Fluorescence Imaging

      • Tracy Lawson, Silvere Vialet-Chabrand
      Pages 121-140

    10. Measurement of O2 Uptake and Evolution in Leaves In Vivo Using Stable Isotopes and Membrane Inlet Mass Spectrometry

      • Steven M. Driever, Neil R. Baker
      Pages 141-154

    11. Using Stable Carbon Isotopes to Study C3 and C4 Photosynthesis: Models and Calculations

      • Nerea Ubierna, Meisha-Marika Holloway-Phillips, Graham D. Farquhar
      Pages 155-196

    12. Liquid-Phase Measurements of Photosynthetic Oxygen Evolution

      • Dmitriy Shevela, Wolfgang P. Schröder, Johannes Messinger
      Pages 197-211

  3. Measuring Photosynthetic Enzyme Abundance and Catalytic Activity

    1. Front Matter

      Pages 213-213
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    2. Quantification of Photosynthetic Enzymes in Leaf Extracts by Immunoblotting

      • J. Alejandro Perdomo, Cristina R. G. Sales, Elizabete Carmo-Silva
      Pages 215-227

    3. Extraction of RuBisCO to Determine Catalytic Constants

      • Douglas J. Orr, Elizabete Carmo-Silva
      Pages 229-238

    4. Spectrophotometric Determination of RuBisCO Activity and Activation State in Leaf Extracts

      • Cristina R. G. Sales, Gustaf E. Degen, Anabela Bernardes da Silva, Elizabete Carmo-Silva
      Pages 239-250
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Keywords

About this book

This user-friendly book provides a range of classical and modern techniques for the study of photosynthesis in a manner accessible to a broad spectrum of researchers. Broken into four sections, it explores the measurement of physiological photosynthetic parameters, quantifying photosynthetic enzyme abundance and catalytic activity, visualizing cellular and sub-cellular phenotypes, and photosynthesis-inspired energy generation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Photosynthesis: Methods and Protocols aims to aid scientists working toward exciting developments in the understanding and application of photosynthesis.

Editors and Affiliations

  • Niceville, USA

    Sarah Covshoff

Bibliographic Information

  • Book Title: Photosynthesis

  • Book Subtitle: Methods and Protocols

  • Editors: Sarah Covshoff

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7786-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7785-7Published: 06 July 2018

  • Softcover ISBN: 978-1-4939-9285-0Published: 10 December 2019

  • eBook ISBN: 978-1-4939-7786-4Published: 05 July 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 355

  • Number of Illustrations: 23 b/w illustrations, 47 illustrations in colour

  • Topics: Plant Sciences

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