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Single Cell Methods

Sequencing and Proteomics

  • Book
  • © 2019

Overview

Editors:
  1. Valentina Proserpio

    1. Department of Life Sciences and System Biology, University of Turin, Italian Institute for Genomic Medicine, IIGM Turin, Torino, Italy

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1979)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. Lab Setup and Tissue Preparation

    1. Front Matter

      Pages 1-1
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    2. Setting Up a Single-Cell Genomic Laboratory

      • Lira Mamanova
      Pages 3-8

    3. Tissue Handling and Dissociation for Single-Cell RNA-Seq

      • Felipe A. Vieira Braga, Ricardo J. Miragaia
      Pages 9-21

  3. Single Cell Trancriptomic Analysis

    1. Front Matter

      Pages 23-23
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    2. Full-Length Single-Cell RNA Sequencing with Smart-seq2

      • Simone Picelli
      Pages 25-44

    3. CEL-Seq2—Single-Cell RNA Sequencing by Multiplexed Linear Amplification

      • Itai Yanai, Tamar Hashimshony
      Pages 45-56

    4. Single-Cell RNA-Seq by Multiple Annealing and Tailing-Based Quantitative Single-Cell RNA-Seq (MATQ-Seq)

      • Kuanwei Sheng, Chenghang Zong
      Pages 57-71

    5. Single-Cell RNA Sequencing with Drop-Seq

      • Josephine Bageritz, Gianmarco Raddi
      Pages 73-85

    6. Chromium 10× Single-Cell 3′ mRNA Sequencing of Tumor-Infiltrating Lymphocytes

      • Marco De Simone, Grazisa Rossetti, Massimiliano Pagani
      Pages 87-110

    7. Seq-Well: A Sample-Efficient, Portable Picowell Platform for Massively Parallel Single-Cell RNA Sequencing

      • Toby P. Aicher, Shaina Carroll, Gianmarco Raddi, Todd Gierahn, Marc H. Wadsworth II, Travis K. Hughes et al.
      Pages 111-132

    8. Single-Cell Tagged Reverse Transcription (STRT-Seq)

      • Kedar Nath Natarajan
      Pages 133-153

    9. Single-Cell RNA-Sequencing of Peripheral Blood Mononuclear Cells with ddSEQ

      • Shaheen Khan, Kelly A. Kaihara
      Pages 155-176

    10. High-Throughput Single-Cell Real-Time Quantitative PCR Analysis

      • Liora Haim-Vilmovsky
      Pages 177-183

    11. Single-Cell Dosing and mRNA Sequencing of Suspension and Adherent Cells Using the PolarisTM System

      • Chad D. Sanada, Aik T. Ooi
      Pages 185-195

    12. Targeted TCR Amplification from Single-Cell cDNA Libraries

      • Shuqiang Li, Kenneth J. Livak
      Pages 197-224

  4. Single Cell Genomic and Epigenomic Analysis

    1. Front Matter

      Pages 225-225
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    2. Sequencing the Genomes of Single Cells

      • Veronica Gonzalez-Pena, Charles Gawad
      Pages 227-234

    3. Studying DNA Methylation in Single-Cell Format with scBS-seq

      • Natalia Kunowska
      Pages 235-250

    4. Single-Cell 5fC Sequencing

      • Chenxu Zhu, Yun Gao, Jinying Peng, Fuchou Tang, Chengqi Yi
      Pages 251-267
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Keywords

About this book

This volume provides a comprehensive overview for investigating biology at the level of individual cells. Chapters are organized into eight parts detailing a single-cell lab, single cell DNA-seq, RNA-seq, single cell proteomic and epigenetic, single cell multi-omics, single cell screening, and single cell live imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Single Cell Methods: Sequencing and Proteomics aims to make each experiment easily reproducible in every lab.



   


Editors and Affiliations

  • Department of Life Sciences and System Biology, University of Turin, Italian Institute for Genomic Medicine, IIGM Turin, Torino, Italy

    Valentina Proserpio

About the editor

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Bibliographic Information

  • Book Title: Single Cell Methods

  • Book Subtitle: Sequencing and Proteomics

  • Editors: Valentina Proserpio

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9240-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9239-3Published: 27 April 2019

  • Softcover ISBN: 978-1-4939-9242-3Published: 26 September 2020

  • eBook ISBN: 978-1-4939-9240-9Published: 26 April 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 459

  • Number of Illustrations: 9 b/w illustrations, 78 illustrations in colour

  • Topics: Cell Biology

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