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Clinical Applications of Capillary Electrophoresis

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Terry M. Phillips

    1. Department of Pharmaceutics, School of Pharmacy, Virginia Commonwealth University, Richmond, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1972)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xii
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  2. Overview

    1. Front Matter

      Pages 1-1
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    2. An Overview of CE in Clinical Analysis

      • David S. Hage
      Pages 3-11

  3. Applications in Clinical Chemistry

    1. Front Matter

      Pages 13-13
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    2. Rapid and Sensitive Determination of Branched-Chain Amino Acids in Human Plasma by Capillary Electrophoresis with Contactless Conductivity Detection for Physiological Studies

      • Petr Tůma
      Pages 15-24

    3. Monitorization of α1-Acid Glycoprotein Deglycosylation Using SU-8 Microchips Electrophoresis with LIF Detection

      • María del Mar Barrios-Romero, Agustín G. Crevillén, Angel Puerta, Mercedes de Frutos, José Carlos Diez-Masa
      Pages 25-39

    4. Glycoform Analysis of Alpha1-Acid Glycoprotein by Capillary Electrophoresis Using Electrophoretic Injection

      • Chenhua Zhang, William Clarke, David S. Hage
      Pages 41-56

    5. On-Line Immunoaffinity Solid-Phase Extraction Capillary Electrophoresis-Mass Spectrometry for the Analysis of Serum Transthyretin

      • Roger Pero-Gascon, Laura Pont, Victoria Sanz-Nebot, Fernando Benavente
      Pages 57-76

    6. Measurement of Neutral and Sialylated IgG n-Glycome at Asn-297 by CE-LIF to Assess Hypogalactosylation in Rheumatoid Arthritis

      • Christian Schwedler, Véronique Blanchard
      Pages 77-93

    7. The Control of Glucose and Lactate Levels in Nutrient Medium After Cell Incubation and in Microdialysates of Human Adipose Tissue by Capillary Electrophoresis with Contactless Conductivity Detection

      • Petr Tůma
      Pages 95-108

    8. Flow-Induced Dispersion Analysis (FIDA) for Protein Quantification and Characterization

      • Morten E. Pedersen, Jesper Østergaard, Henrik Jensen
      Pages 109-123

  5. Application in Metabolomics

    1. Front Matter

      Pages 137-137
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    2. New Advances for Newborn Screening of Inborn Errors of Metabolism by Capillary Electrophoresis-Mass Spectrometry (CE-MS)

      • Meera Shanmuganathan, Philip Britz-McKibbin
      Pages 139-163

    3. Capillary Electrophoresis-Mass Spectrometry for Metabolic Profiling of Biomass-Limited Samples

      • Wei Zhang, Thomas Hankemeier, Rawi Ramautar
      Pages 165-172
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Keywords

About this book

This second edition volume provides a valuable source of information on the application of capillary electrophoresis (CE) and the many different aspects of clinical medicine. Chapters divided into seven parts focus on applications in clinical chemistry and small molecule analysis, applications in drug analysis, examples of CE applied to metabolomics, application in pediatrics, CE analysis on oncology, and CE analysis in virology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, Clinical Applications of Capillary Electrophoresis: Methods and Protocols, Second Edition aims to become a resource not only for clinical chemists, but also physicians and scientists who wish to apply these techniques to diagnosis and clinical research.

Editors and Affiliations

  • Department of Pharmaceutics, School of Pharmacy, Virginia Commonwealth University, Richmond, USA

    Terry M. Phillips

Bibliographic Information

  • Book Title: Clinical Applications of Capillary Electrophoresis

  • Book Subtitle: Methods and Protocols

  • Editors: Terry M. Phillips

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9213-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9212-6Published: 07 March 2019

  • eBook ISBN: 978-1-4939-9213-3Published: 07 March 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 273

  • Number of Illustrations: 23 b/w illustrations, 51 illustrations in colour

  • Topics: Biochemistry, general

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