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Streptococcus pneumoniae

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Federico Iovino

    1. Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Bioclinicum, Stockholm, Sweden

    View editor publications

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1968)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xv
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  2. Cultivation of Streptococcus pneumoniae In Vitro

    1. Front Matter

      Pages 1-1
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    2. Optimal Conditions for Streptococcus pneumoniae Culture: In Solid and Liquid Media

      • Norma Suárez, Esther Texeira
      Pages 3-10

  3. Microscopy Techniques to Study the Biology of Streptococcus pneumoniae and Pneumococcal Interactions with the Host

    1. Front Matter

      Pages 11-11
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    2. Electron Microscopy to Study the Fine Structure of the Pneumococcal Cell

      • Sven Hammerschmidt, Manfred Rohde
      Pages 13-33

    3. Immunofluorescent Staining and High-Resolution Microscopy to Study the Pneumococcal Cell

      • Federico Iovino, Birgitta Henriques-Normark
      Pages 35-39

    4. Construction of Fluorescent Pneumococci for In Vivo Imaging and Labeling of the Chromosome

      • Morten Kjos
      Pages 41-51

    5. High-Resolution and Super-Resolution Immunofluorescent Microscopy Ex Vivo to Study Pneumococcal Interactions with the Host

      • Federico Iovino, Birgitta Henriques-Normark
      Pages 53-59

  4. The Genetics of Streptococcus pneumoniae

    1. Front Matter

      Pages 61-61
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    2. Natural Genetic Transformation: A Direct Route to Easy Insertion of Chimeric Genes into the Pneumococcal Chromosome

      • Isabelle Mortier-Barrière, Nathalie Campo, Mathieu A. Bergé, Marc Prudhomme, Patrice Polard
      Pages 63-78

    3. Gene Expression Analysis in the Pneumococcus

      • Rory A. Eutsey, Carol A. Woolford, Surya D. Aggarwal, Rolando A. Cuevas, N. Luisa Hiller
      Pages 79-88

    4. Transcriptional Knockdown in Pneumococci Using CRISPR Interference

      • Morten Kjos
      Pages 89-98

  5. The Proteome and Proteomics of Streptococcus pneumoniae

    1. Front Matter

      Pages 99-99
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    2. Protein Expression Analysis by Western Blot and Protein–Protein Interactions

      • María Dolores Cima-Cabal, Fernando Vazquez, Juan R. de los Toyos, María del Mar García-Suárez
      Pages 101-111

    3. Mass Spectrometry to Study the Bacterial Proteome from a Single Colony

      • Jianwei Zhou, Lu Zhang, Huixia Chuan, Angela Sloan, Raymond Tsang, Keding Cheng
      Pages 113-121

    4. Bead-Based Flow-Cytometric Cell Counting of Live and Dead Bacteria

      • Fang Ou, Cushla McGoverin, Joni White, Simon Swift, Frédérique Vanholsbeeck
      Pages 123-134

  6. Streptococcus pneumoniae-Host Interactions: In Vitro and In Vivo Models

    1. Front Matter

      Pages 135-135
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    2. In Vitro Adhesion, Invasion, and Transcytosis of Streptococcus pneumoniae with Host Cells

      • Terry Brissac, Carlos J. Orihuela
      Pages 137-146

    3. Growing and Characterizing Biofilms Formed by Streptococcus pneumoniae

      • Yashuan Chao, Caroline Bergenfelz, Anders P. Hakansson
      Pages 147-171

    4. In Vivo Mouse Models to Study Pneumococcal Host Interaction and Invasive Pneumococcal Disease

      • Federico Iovino, Vicky Sender, Birgitta Henriques-Normark
      Pages 173-181
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Keywords

About this book

This volume looks at the newest methods, materials, equipment, and technologies developed to study the cell biology of the pneumococcus. The chapters, which cover a wide range of topics, are organized into six parts: Part one discusses the cultivation of Streptococcus pneumoniae in vitro; Part Two talks about the microscopy techniques used to study the biology of Streptococcus pneumoniae and pneumococcal interactions with the host; Parts Three and Four describe the genetics, proteome, and proteomics of Streptococcus pneumoniae; Part Five explains in vitro and in vivo models of Streptococcus pneumoniae-host interactions; and Part Six looks at the factors of public health, epidemiology, and biostatistics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Streptococcus pneumoniae: Methods and Protocols is a valuable resource for both novice and expert researchers who want to expand their studies into new areas and new systems in this continuously evolving field.


Reviews

“The audience includes students, researchers, and microbiologists, as well as others in the fields of public health, epidemiology, and biostatistics related to Streptococcus pneumoniae. ... This is an excellent book on Streptococcus pneumoniae, with additional coverage of the related public health, epidemiology, and biostatistics aspects. I am sure that the information in this book will be of much help and interest to the intended audience.” (Omer Iqbal, Doody's Book Reviews, December 7, 2019)

Editors and Affiliations

  • Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Bioclinicum, Stockholm, Sweden

    Federico Iovino

Bibliographic Information

  • Book Title: Streptococcus pneumoniae

  • Book Subtitle: Methods and Protocols

  • Editors: Federico Iovino

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9199-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9198-3Published: 01 April 2019

  • eBook ISBN: 978-1-4939-9199-0Published: 30 March 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 227

  • Number of Illustrations: 11 b/w illustrations, 26 illustrations in colour

  • Topics: Infectious Diseases

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