Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1957)
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Table of contents (25 protocols)
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Front Matter
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Methods to Study Roles of β-Arrestins in GPCR Regulation
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Front Matter
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Methods to Study Scaffolding Functions of β-Arrestins
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Front Matter
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Methods to Analyse Ligand Bias
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Front Matter
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About this book
This volume looks at various approaches to study the pleiotropic roles of b-arrestins (b-arrs) in the control of signal transduction, and the resulting cellular and in vivo consequences that arise. The chapters in this book cover diverse topics around b-arrs such as their established roles in GPCR regulation and trafficking; regulatory scaffolding functions of b-arrs in MAPK signaling, cAMP hydrolysis and cytoskeletal dynamics; proteomic analysis of the b-arr interactome; mathematical modelling of b-arr signaling networks; functional selectivity involving biased ligands; nucleocytoplasmic trafficking and primary cilia-associated functions of b-arrs; conformational plasticity of b-arrs; and the roles of b-arrs in allergic inflammation, Type 2 Diabetes, Parkinson’s disease, and cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents,step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Beta-Arrestins: Methods and Protocols is a valuable resource for researchers interested in learning more about the function and regulation of b-arrestins.
Editors and Affiliations
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Institut Cochin, INSERM U1016, Paris, France
Mark G. H. Scott
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Department of Medicine, Research Institute of the McGill, University Health Center (RI-MUHC), McGill University, Montreal, Canada
Stéphane A. Laporte
Bibliographic Information
Book Title: Beta-Arrestins
Book Subtitle: Methods and Protocols
Editors: Mark G. H. Scott, Stéphane A. Laporte
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-9158-7
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019
Hardcover ISBN: 978-1-4939-9157-0Published: 28 March 2019
eBook ISBN: 978-1-4939-9158-7Published: 27 March 2019
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 409
Number of Illustrations: 19 b/w illustrations, 60 illustrations in colour
Topics: Protein Science, Cell Biology, Receptors