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  • Book
  • © 2019

Calcium-Binding Proteins of the EF-Hand Superfamily

From Basics to Medical Applications

Editors:

  1. Claus W. Heizmann

    1. Division of Clinical Chemistry and Biochemistry, Department of Pediatrics, University of Zürich, Zürich, Switzerland

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1929)

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Table of contents (48 protocols)

  1. Front Matter

    Pages i-xix
    PDF

  2. Data Analysis and Modern Technologies to Study Calcium-Binding and Signaling in Cells

    1. Front Matter

      Pages 1-1
      PDF

    2. Using Data Mining to Explore Calmodulin Bibliography

      • Jacques Haiech, Marie-Claude Kilhoffer
      Pages 3-14

    4. High-Resolution Calcium Imaging Method for Local Calcium Signaling

      • Björn-Philipp Diercks, René Werner, Daniel Schetelig, Insa M. A. Wolf, Andreas H. Guse
      Pages 27-39

    8. Cellular Ca2+-Responding Nanoluciferase Reporter Gene System Directed by Tandemly Repeated Pseudo-palindromic NFAT-Response Elements

      • Wei Zhang, Terunao Takahara, Takuya Achiha, Hideki Shibata, Masatoshi Maki
      Pages 95-109

    9. Designing Calcium-Binding Proteins for Molecular MR Imaging

      • Mani Salarian, Shenghui Xue, Oluwatosin Y. Ibhagui, Jenny J. Yang
      Pages 111-125

    10. Coordination to Divalent Cations by Calcium-Binding Proteins

      • Masayuki Nara, Hisayuki Morii, Masaru Tanokura
      Pages 127-134

    11. Chaperoning Against Amyloid Aggregation: Monitoring In Vitro and In Vivo

      • Ravichandran Vignesh, Gopala Krishna Aradhyam
      Pages 135-154

  3. The Superfamily of Calcium-Binding Proteins Characterized by the EF-Hand Structural Motif

    1. Front Matter

      Pages 155-155
      PDF

    3. Gene Transfer of Calcium-Binding Proteins into Adult Cardiac Myocytes

      • Brian R. Thompson, Houda Cohen, Addeli Bez Batti Angulski, Joseph M. Metzger
      Pages 187-205

    4. Expression and Purification of Calmodulin for NMR and Other Biophysical Applications

      • Benjamin M. M. Grant, Christopher B. Marshall, Mitsuhiko Ikura
      Pages 207-221

    6. Calpain Purification Through Calpastatin and Calcium: Strategy and Procedures

      • Hung Huy Nguyen, Peter Tompa, Kris Pauwels
      Pages 233-244

    7. Characterization of the EF-Hand Calcium-Binding Domains of Human Plastins

      • Oleg Khassan, Katharine V. Jensen, Andrew G. Woodman, Hans J. Vogel, Hiroaki Ishida
      Pages 245-260

    8. Expression and Characterization of MICU2, a Ca2+ Sensor Protein

      • Wenping Wu, Jimin Zheng, Zongchao Jia
      Pages 261-273
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About this book

This detailed volume explores protocols for studying the many facets of Ca2+-imaging, Ca2+-signaling, and Ca2+-binding along with background information on the principles and application of these techniques. The content of the book delves into 48 chapters including subjects such as data analysis and modern technologies to study calcium-binding and signaling in cells, the superfamily of calcium-binding proteins characterized by the EF-hand structural motif, as well as their use as diagnostic and prognostic biomarkers in Laboratory Medicine and novel therapeutic drug targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and comprehensive, Calcium-Binding Proteins ofthe EF-Hand Superfamily: From Basics to Medical Applications presents state-of-the-art, lab-based methods and easy-to-follow protocols for daily use, making it interesting for basic and medical researchers, cell- and molecular biologists, clinicians, clinical chemists, and the diagnostic industry.

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Keywords

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Editors and Affiliations

  • Division of Clinical Chemistry and Biochemistry, Department of Pediatrics, University of Zürich, Zürich, Switzerland

    Claus W. Heizmann

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Bibliographic Information

  • Book Title: Calcium-Binding Proteins of the EF-Hand Superfamily

  • Book Subtitle: From Basics to Medical Applications

  • Editors: Claus W. Heizmann

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9030-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9029-0Published: 02 February 2019

  • eBook ISBN: 978-1-4939-9030-6Published: 01 February 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIX, 779

  • Number of Illustrations: 58 b/w illustrations, 117 illustrations in colour

  • Topics: Protein Science, Laboratory Medicine

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Buy it now

eBook USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

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