Skip to main content
SpringerLink
Log in

Calcium Signalling

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Anna Raffaello

    1. Department of Biomedical Sciences, University of Padua, Padua, Italy

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Denis Vecellio Reane

    1. Department of Biomedical Sciences, University of Padua, Padua, Italy

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1925)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 44.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 59.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (21 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. High-Throughput Screening Using Photoluminescence Probe to Measure Intracellular Calcium Levels

    • Simona Feno, Giulia Di Marco, Agnese De Mario, Halenya Monticelli, Denis Vecellio Reane
    Pages 1-14

  3. Exploiting Cameleon Probes to Investigate Organelles Ca2+ Handling

    • Luisa Galla, Paola Pizzo, Elisa Greotti
    Pages 15-30

  4. Measuring Ca2+ Levels in Subcellular Compartments with Genetically Encoded GFP-Based Indicators

    • Mattia Vicario, Tito Calì
    Pages 31-42

  5. Methods to Measure Intracellular Ca2+ Concentration Using Ca2+-Sensitive Dyes

    • Sofia Zanin, Elisa Lidron, Rosario Rizzuto, Giorgia Pallafacchina
    Pages 43-58

  6. MCU Regulation in Lipid Bilayer and Electrophysiological Recording

    • Vanessa Checchetto, Ildikò Szabò
    Pages 59-63

  7. Electrophysiological Characterization of Calcium-Permeable Channels Using Planar Lipid Bilayer

    • Vanessa Checchetto, Ildikò Szabò
    Pages 65-73

  8. Patch-Clamp Analysis of the Mitochondrial Calcium Uniporter

    • Vivek Garg, Yuriy Y. Kirichok
    Pages 75-86

  9. In Vivo Light Sheet Fluorescence Microscopy of Calcium Oscillations in Arabidopsis thaliana

    • Neli Romano Armada, Fabrizio Gandolfo Doccula, Alessia Candeo, Gianluca Valentini, Alex Costa, Andrea Bassi
    Pages 87-101

  10. Ex Vivo Measurements of Ca2+ Transients in Intracellular Compartments of Skeletal Muscle Fibers by Means of Genetically Encoded Probes

    • Gaia Gherardi, Cristina Mammucari
    Pages 103-109

  11. Imaging Intracellular Ca2+ in Cardiomyocytes with Genetically Encoded Fluorescent Probes

    • Antonio Campo, Marco Mongillo
    Pages 111-125

  12. In Vivo Monitoring of Ca2+ Uptake into Subcellular Compartments of Mouse Skeletal Muscle

    • Rüdiger Rudolf, Sofie Trajanovska, David Grant Allen, Tullio Pozzan
    Pages 127-142

  13. TRPML1-/TFEB-Dependent Regulation of Lysosomal Exocytosis

    • Simone Di Paola, Diego L. Medina
    Pages 143-144

  14. Ca2+-Dependent Regulation of TFEB and Lysosomal Function

    • Simone Di Paola, Diego L. Medina
    Pages 145-155

  15. Employing Transgenic Parasite Strains to Study the Ca2+ Dynamics in the Human Malaria Parasite Plasmodium falciparum

    • Lucas Borges-Pereira, Célia R. S. Garcia
    Pages 157-162

  16. Calcium Imaging of Store-Operated Calcium (Ca2+) Entry (SOCE) in HEK293 Cells Using Fura-2

    • Martin Johnson
    Pages 163-172

  17. Optogenetic Interneuron Stimulation and Calcium Imaging in Astrocytes

    • Gabriele Losi, Anna Maria Lia, Marta Gomez-Gonzalo, Micaela Zonta, Giorgio Carmignoto
    Pages 173-182

  18. Measuring Calcium and ROS by Genetically Encoded Protein Sensors and Fluorescent Dyes

    • Christine S. Gibhardt, Adina Vultur, Ivan Bogeski
    Pages 183-196

  19. Assessing Calcium-Stimulated Mitochondrial Bioenergetics Using the Seahorse XF96 Analyzer

    • Jennifer Wettmarshausen, Fabiana Perocchi
    Pages 197-222

  20. Determination of ATP, ADP, and AMP Levels by Reversed-Phase High-Performance Liquid Chromatography in Cultured Cells

    • Michela Menegollo, Isabella Tessari, Luigi Bubacco, Gyorgy Szabadkai
    Pages 223-232
Back to top

Keywords

About this book

This volume looks at the latest developments in studying calcium signaling. The chapters in this book discuss topics such as in vitro and in vivo analyses in model systems; methods to measure Ca2+ in different subcellular compartments; single Ca2+ channels; methods to measure cellular ROS and ATP; and the functionality of the ATP synthase. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Calcium Signalling: Methods and Protocols is a valuable resource that covers both conceptual and methodological viewpoints to aid beginners and experts in furthering their studies in the developing field of calcium homeostasis research.


Editors and Affiliations

  • Department of Biomedical Sciences, University of Padua, Padua, Italy

    Anna Raffaello, Denis Vecellio Reane

Bibliographic Information

  • Book Title: Calcium Signalling

  • Book Subtitle: Methods and Protocols

  • Editors: Anna Raffaello, Denis Vecellio Reane

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9018-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9017-7Published: 23 January 2019

  • eBook ISBN: 978-1-4939-9018-4Published: 23 January 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 246

  • Number of Illustrations: 9 b/w illustrations, 39 illustrations in colour

  • Topics: Biochemistry, general

Publish with us

Policies and ethics

Back to top

Search

Navigation