Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1917)
Buy it now
Buying options
Tax calculation will be finalised at checkout
Other ways to access
This is a preview of subscription content, log in via an institution to check for access.
Table of contents (27 protocols)
-
Front Matter
-
Review on Plant DNA Repair and Genome Editing
-
Front Matter
-
-
CRISPR Design and Mutation Analysis
-
Front Matter
-
-
Multiplexed CRISPR-Cas9 Systems
-
Front Matter
-
-
CRISPR-Cas9 Editing in Dicots
-
Front Matter
-
About this book
This volume provides readers with wide-ranging coverage of CRISPR systems and their applications in various plant species. The chapters in this book discuss topics such as plant DNA repair and genome editing; analysis of CRISPR-induced mutations; multiplexed CRISPR/Cas9 systems; CRISPR-Cas12a (Cpf1) editing systems; and non-agrobacterium based CRISPR delivery systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and thorough, Plant Genome Editing with CRISPR Systems: Methods and Protocols is a valuable resource for any researcher interested in learning about and using CRISPR systems in plants.
Editors and Affiliations
-
Department of Plant Science and Landscape Architecture, University of Maryland College Park, College Park, USA
Yiping Qi
Bibliographic Information
Book Title: Plant Genome Editing with CRISPR Systems
Book Subtitle: Methods and Protocols
Editors: Yiping Qi
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-8991-1
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019
Hardcover ISBN: 978-1-4939-8990-4Published: 05 January 2019
eBook ISBN: 978-1-4939-8991-1Published: 04 January 2019
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XV, 367
Number of Illustrations: 8 b/w illustrations, 65 illustrations in colour