Microfluidic Electrophoresis

1. Front Matter

   Pages i-x

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2. Fabrication of Glass Microfluidic Devices

   • Christopher T. Culbertson, Jay Sibbitts, Kathleen Sellens, Shu Jia

   Pages 1-12

3. Soft Lithography, Molding, and Micromachining Techniques for Polymer Micro Devices

   • Ashis Kumar Sen, Abhishek Raj, Utsab Banerjee, Sk Rameez Iqbal

   Pages 13-54

4. Sample Injection Techniques

   • James M. Karlinsey

   Pages 55-64

5. Sample Preconcentration Protocols in Microfluidic Electrophoresis

   • Fumihiko Kitagawa, Koji Otsuka

   Pages 65-78

6. Microchip Electrophoresis Containing Electrodes for Integrated Electrochemical Detection

   • Lucas Paines Bressan, Dosil Pereira de Jesus, Dulan Bandara Gunasekara, Susan Marie Lunte, José Alberto Fracassi da Silva

   Pages 79-85

7. Micellar Electrokinetic Chromatography

   • Braden C. Giordano, Ronald Siefert, Greg E. Collins

   Pages 87-97

8. Microchip Isotachophoresis: Analysis of Pharmaceuticals

   • Marián Masár, Jasna Hradski

   Pages 99-111

9. Microfluidic Free-Flow Isoelectric Focusing with Real-Time pI Determination

   • Stefan Nagl

   Pages 113-124

10. Nanochannel Gradient Separations

    • Michael A. Startsev, David W. Inglis

    Pages 125-132

11. Paper-Based Electrophoresis Microchip as a Powerful Tool for Bioanalytical Applications

    • Cyro L. S. Chagas, Thiago M. G. Cardoso, Wendell K. T. Coltro

    Pages 133-142

12. Band Broadening Theories in Capillary Electrophoresis

    • Sandip Ghosal

    Pages 143-166

13. Estimating Stream Broadening in Free-Flow Electrophoretic Systems Based on the Method-of-Moments Formulation

    • Debashis Dutta

    Pages 167-195

14. Microchip Electrophoresis Tools for the Analysis of Small Molecules

    • Federico J. V. Gomez, María Fernanda Silva

    Pages 197-206

15. Integrated Microfluidic System for Rapid DNA Fingerprint Analysis: A Miniaturized Integrated DNA Analysis System (MiDAS)—Swab Sample-In to DNA Profile-Out

    • Jianing Yang, Cedric Hurth, Alan Nordquist, Stan Smith, Frederic Zenhausern

    Pages 207-224

16. Achieving Stable Electrospray Ionization Mass Spectrometry Detection from Microfluidic Chips

    • Iulia M. Lazar

    Pages 225-237

17. Microchip-Based Electrophoretic Separations with a Pressure-Driven Backflow

    • Ling Xia, Debashis Dutta

    Pages 239-249

18. Back Matter

    Pages 251-255

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This detailed book provides a set of protocols necessary for the development of a variety of microchip-based electrophoretic assays. It compiles a range of such electrophoretic methods by leading researchers in the field, covering subjects such as microfluidic device fabrication, on-chip sample preparation, theoretical/simulation protocols for assessing these separation methods, as well as common practices followed when applying them to important real world applications. The contents of the book range from protocols for classical assays to those involving pioneering separation techniques recently developed by the scientific community for advancing our analytical capabilities. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and accessible, Microfluidic Electrophoresis: Methods and Protocols serves as an convenient text for academic researchers as well as practicing engineers, biochemists, and analytical laboratory professionals.

• Electrophoretic assays
• Microfluidic devices
• Microchip-based assays
• On-chip sample preparation
• Separation techniques
• Sample preparation
• Electrophoresis

• Department of Chemistry, University of Wyoming, Laramie, USA

  Debashis Dutta

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