Human Monoclonal Antibodies

1. Front Matter

   Pages i-xiv

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2. Human Monoclonal Antibodies: The Benefits of Humanization

   • Herman Waldmann

   Pages 1-10

3. Cancer Immunotherapy: The Dawn of Antibody Cocktails

   • Ilaria Marrocco, Donatella Romaniello, Yosef Yarden

   Pages 11-51

4. IgM Natural Autoantibodies in Physiology and the Treatment of Disease

   • Mahboobeh Fereidan-Esfahani, Tarek Nayfeh, Arthur Warrington, Charles L. Howe, Moses Rodriguez

   Pages 53-81

5. Monoclonal Antibodies for the Treatment of Melanoma: Present and Future Strategies

   • Madhuri Bhandaru, Anand Rotte

   Pages 83-108

6. An Efficient Method to Generate Monoclonal Antibodies from Human B Cells

   • Jenna J. Guthmiller, Haley L. Dugan, Karlynn E. Neu, Linda Yu-Ling Lan, Patrick C. Wilson

   Pages 109-145

7. Isolation of Antigen-Specific, Antibody-Secreting Cells Using a Chip-Based Immunospot Array

   • Hiroyuki Kishi, Tatsuhiko Ozawa, Hiroshi Hamana, Eiji Kobayashi, Atsushi Muraguchi

   Pages 147-162

8. Purification of Human Monoclonal Antibodies and Their Fragments

   • Nicole Ulmer, Sebastian Vogg, Thomas Müller-Späth, Massimo Morbidelli

   Pages 163-188

9. One-Tube Multicolor Flow Cytometry Assay (OTMA) for Comprehensive Immunophenotyping of Peripheral Blood

   • Anna-Jasmina Donaubauer, Paul F. Rühle, Ina Becker, Rainer Fietkau, Udo S. Gaipl, Benjamin Frey

   Pages 189-212

10. Humanization and Simultaneous Optimization of Monoclonal Antibody

    • Taichi Kuramochi, Tomoyuki Igawa, Hiroyuki Tsunoda, Kunihiro Hattori

    Pages 213-230

11. Antibody Fragments Humanization: Beginning with the End in Mind

    • Nicolas Aubrey, Philippe Billiald

    Pages 231-252

12. Antigen-Specific Human Monoclonal Antibodies from Transgenic Mice

    • Susana Magadán Mompó, África González-Fernández

    Pages 253-291

13. Refining the Quality of Monoclonal Antibodies: Grafting Unique Peptide-Binding Site in the Fab Framework

    • Jeremy D. King, John C. Williams

    Pages 293-298

14. Basic Procedures for Detection and Cytotoxicity of Chimeric Antigen Receptors

    • Keichiro Mihara, Tetsumi Yoshida, Joyeeta Bhattacharyya

    Pages 299-306

15. Rapid Chimerization of Antibodies

    • Koji Hashimoto, Kohei Kurosawa, Hidetaka Seo, Kunihiro Ohta

    Pages 307-317

16. Phage Display Technology for Human Monoclonal Antibodies

    • Marco Dal Ferro, Serena Rizzo, Emanuela Rizzo, Francesca Marano, Immacolata Luisi, Olga Tarasiuk et al.

    Pages 319-338

17. Recombinant Antibody Selections by Combining Phage and Yeast Display

    • Fortunato Ferrara, Maria Felicia Soluri, Daniele Sblattero

    Pages 339-352

18. Epitope Mapping via Phage Display from Single-Gene Libraries

    • Viola Fühner, Philip Alexander Heine, Kilian Johannes Carl Zilkens, Doris Meier, Kristian Daniel Ralph Roth, Gustavo Marçal Schmidt Garcia Moreira et al.

    Pages 353-375

19. A High-Throughput Magnetic Nanoparticle-Based Semi-Automated Antibody Phage Display Biopanning

    • Angela Chiew Wen Ch’ng, Azimah Ahmad, Zoltán Konthur, Theam Soon Lim

    Pages 377-400

20. A Binding Potency Assay for Pritumumab and Ecto-Domain Vimentin

    • Ivan Babic, Santosh Kesari, Mark C. Glassy

    Pages 401-415

This second edition volume expands on the previous edition with descriptions of recent developments in the field. The chapters in this book cover topics such as monoclonal antibodies for the treatment of melanoma; production and purification of human monoclonal antibodies; humanization and optimization of monoclonal antibodies; rapid chimerization of monoclonal antibodies; epitope mapping via phage display from single gene libraries; recombinant antibodies made by combining phage and yeast display selections; production of stabilized antibody fragments in the E. coli bacterial cytoplasm and transfected mammalian cells; and analysis of CAR T cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Uniqueand thorough, Human Monoclonal Antibodies: Methods and Protocols, Second Edition is a valuable tool for novice and expert researchers interested in learning more about this evolving field.

• transgenic mice
• phage display
• Alzheimer’s disease
• melanoma
• T-cells
• antibodies

• Department of Pathology, The Hebrew University, Hadassah Medical School, Jerusalem, Israel

  Michael Steinitz

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