Blood-Brain Barrier

1. Front Matter

   Pages i-xiii

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2. An Overview of the Blood-Brain Barrier

   • Tatiana Barichello, Allan Collodel, Rodrigo Hasbun, Rodrigo Morales

   Pages 1-8

3. Methods of Delivering Molecules Through the Blood-Brain Barrier for Brain Diagnostics and Therapeutics

   • Brian M. Kopec, Kavisha R. Ulapane, Mario E. G. Moral, Teruna J. Siahaan

   Pages 9-43

4. Culturing of Rodent Brain Microvascular Endothelial Cells for In Vitro Modeling of the Blood-Brain Barrier

   • Malgorzata Burek, Carola Y. Förster

   Pages 45-54

5. In Vitro BBB Models: Working with Static Platforms and Microfluidic Systems

   • Mohammad A. Kaisar, Vinay V. Abhyankar, Luca Cucullo

   Pages 55-70

6. In Vitro Cell Models of the Human Blood-Brain Barrier: Demonstrating the Beneficial Influence of Shear Stress on Brain Microvascular Endothelial Cell Phenotype

   • Keith D. Rochfort, Philip M. Cummins

   Pages 71-98

7. Transepithelial/Transendothelial Electrical Resistance (TEER) to Measure the Integrity of Blood-Brain Barrier

   • Balaji Srinivasan, Aditya Reddy Kolli

   Pages 99-114

8. Cell-Penetrating Peptides as Theranostics Against Impaired Blood-Brain Barrier Permeability: Implications for Pathogenesis and Therapeutic Treatment of Neurodegenerative Disease

   • Swapna Bera, Anirban Bhunia

   Pages 115-136

9. Microbial Translocation of the Blood-Brain Barrier

   • Charles T. Spencer, Mireya G. Ramos Muniz

   Pages 137-161

10. Transport Across the Choroid Plexus: How to Culture Choroid Plexus Cells and Establish a Functional Assay System

    • Sen Takeda, Keishi Narita

    Pages 163-173

11. Drosophila as a Model to Study the Blood-Brain Barrier

    • Cameron R. Love, Brigitte Dauwalder

    Pages 175-185

12. Zebrafish (Danio rerio) as a Viable Model to Study the Blood-Brain Barrier

    • Tianzhi Yang, Shuhua Bai

    Pages 187-196

13. Evans Blue-Albumin as a Marker to Evaluate Blood-Brain Barrier Integrity in Neonatal and Adult Rodents

    • Fabricia Petronilho, Julia L. Goldman, Tatiana Barichello

    Pages 197-203

14. Experimental Tools to Study the Regulation and Function of the Choroid Plexus

    • Isabel Gonçalves, Telma Quintela, Ana Catarina Duarte, Peter Hubbard, Graça Baltazar, Christian Schwerk et al.

    Pages 205-230

15. Techniques for Evaluating Efflux Transport of Radiolabeled Drugs and Compounds from the Cerebrospinal Fluid Across the Blood-Cerebrospinal Fluid Barrier

    • Shin-ichi Akanuma, Yoshiyuki Kubo, Ken-ichi Hosoya

    Pages 231-248

16. In Vivo Analysis to Study Transport Across the Blood-Retinal Barrier

    • Yoshiyuki Kubo, Shin-ichi Akanuma, Ken-ichi Hosoya

    Pages 249-265

17. Increasing BBB Permeability via Focused Ultrasound: Current Methods in Preclinical Research

    • Dallan McMahon, Charissa Poon, Kullervo Hynynen

    Pages 267-297

18. Evaluation of Blood–Brain Barrier Permeability and Integrity in Juvenile Rodents: Dynamic Contrast-Enhanced (DCE), Magnetic Resonance Imaging (MRI), and Evans Blue Extravasation

    • Trish Domi, Faraz Honarvar, Andrea Kassner

    Pages 299-314

19. Recording Leukocyte Rolling and Adhesion on Meningeal Vessels by Intravital Microscopy

    • Aline Silva de Miranda, Thiago Macedo Cordeiro, Milene Alvarenga Rachid, Antônio Lúcio Teixeira

    Pages 315-327

20. Molecular Imaging of Blood–Brain Barrier Permeability in Preclinical Models Using PET and SPECT

    • Vijayasree V. Giridharan, Tatiana Barichello, Sudhakar Selvaraj

    Pages 329-342

This volume focuses on experimental research with applicable models to study physiology, biochemistry, and molecular biology of the blood-brain barrier (BBB). This book is organized into six parts: Part One is an overview of the physiology of BBB; Part Two explores in vitro cell models to study the BBB; Part Three discusses techniques in vivo and ex vivo models to evaluate BBB in Drosophila melanogaster, Zebrafish, and rodents; Part Four looks at permeability, influx, efflux transportation, and drug delivery through the BBB; Part Five talks about various invasive and non-invasive imaging techniques to study BBB; and Part Six describes how molecular biomarkers are used to look at the integrity or dysfunction of the BBB. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. 



Cutting-edge and thorough, Blood-Brain Barrier is a valuable resource to aid both novice and experienced investigators with performing experiments using new and classic translational approaches.

• Neuronal progenitor cell
• TEER
• Sodium fluorescein
• choroid plexuses
• drug transport

• Department of Psychiatry & Behavioral Sciences, The University of Texas Health Science Center at Houston (UTHealth), Houston, USA

  Tatiana Barichello

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