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Nanotoxicity

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Qunwei Zhang

    1. Department of Environmental and Occupational Health Sciences, School of Public Health and Information Sciences, University of Louisville, Louisville, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1894)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. In Vitro Methods for Assessing Nanoparticle Toxicity

    • Dustin T. Savage, J. Zach Hilt, Thomas D. Dziubla
    Pages 1-29

  3. Methods for Assessing Mast Cell Responses to Engineered Nanomaterial Exposure

    • Nasser B. Alsaleh, Jared M. Brown
    Pages 31-45

  4. Preparation and Utilization of a 3D Human Liver Microtissue Model for Nanotoxicological Assessment

    • Ali Kermanizadeh
    Pages 47-55

  5. Deriving Neural Cells from Pluripotent Stem Cells for Nanotoxicity Testing

    • Yiling Hong, Nymph Chan, Aynun N. Begum
    Pages 57-72

  6. Methods for Evaluation of Ovarian Granulosa Cells with Exposure to Nanoparticles

    • Jin Liu, Wenchang Zhang
    Pages 73-81

  7. In Vitro Approaches for Assessing the Genotoxicity of Nanomaterials

    • Maria Dusinska, Espen Mariussen, Elise Rundén-Pran, Alexandra Misci Hudecova, Elisabeth Elje, Alena Kazimirova et al.
    Pages 83-122

  8. Measurement of Nanoparticle-Induced Mitochondrial Membrane Potential Alterations

    • Salik Hussain
    Pages 123-131

  9. Application of Gelatin Zymography in Nanotoxicity Research

    • Yue Zhang, Rong Wan, Qunwei Zhang, Yiqun Mo
    Pages 133-143

  10. Determination of Phosphorylated Histone H2AX in Nanoparticle-Induced Genotoxic Studies

    • Rong Wan, Yiqun Mo, Ruirui Tong, Meiqin Gao, Qunwei Zhang
    Pages 145-159

  11. Analysis of Nanomaterial Toxicity by Western Blot

    • Gao Long, Yiqun Mo, Qunwei Zhang, Mizu Jiang
    Pages 161-169

  12. Quantification of Mitochondrial Genome Transcription in Male Reproductive Cells by Real-Time PCR

    • Cheng Xu, Qian Liu, Jin Xu, Aihua Gu
    Pages 171-180

  13. DNA Methylation Analysis

    • Lingfang Feng, Jianlin Lou
    Pages 181-227

  14. Evaluation of Nrf2 with Exposure to Nanoparticles

    • Fuli Zheng, Huangyuan Li
    Pages 229-246

  15. Application of Electron Microscopes in Nanotoxicity Assessment

    • Junzhe Zhang, Xiao He, Michael T. Tseng
    Pages 247-269

  16. Metabolic Fingerprinting of Bacteria Exposed to Nanomaterials, Using Online Databases, NMR, and High-Resolution Mass Spectrometry

    • Theodoros G. Chatzimitakos, Constantine D. Stalikas
    Pages 271-280

  17. Methods to Quantify Nanomaterial Association with, and Distribution Across, the Blood–Brain Barrier In Vivo

    • Robert A. Yokel
    Pages 281-299

  18. Evaluation of Genotoxicity of Nanoparticles in Mouse Models

    • Sameera Nallanthighal, Ramune Reliene
    Pages 301-312

  19. Evaluation of Pulmonary Toxicity of Nanoparticles by Bronchoalveolar Lavage

    • Yiqun Mo, Yue Zhang, Qunwei Zhang
    Pages 313-322

  20. Pulmonary Function Testing in Animals

    • Gary W. Hoyle, Connie F. Schlueter, Sadiatu Musah
    Pages 323-330
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Keywords

About this book

This volume explores the latest techniques used to investigate nanotoxicity. The protocols covered in this book range from routine nanotoxicity assays to genomic and proteomic approaches in vivo and in vitro. The chapters discuss specific topics such as preparation and utilization of 3D human liver microtissue models; assessment of ovarian granulosa cells with exposure to nanoparticles; DNA methylation analysis; evaluation of genotoxicity of nanoparticles in mouse models; and studying nanotoxicity using embryonic zebrafish. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Cutting-edge and practical, Nanotoxicity: Methods and Protocols is a valuable resource for researchers and postgraduate students in the fields of toxicology, environmental science, pharmacological sciences, and clinical medicine. This volume also serves as a starting point for researchers interested in learning more about this developing field.


Editors and Affiliations

  • Department of Environmental and Occupational Health Sciences, School of Public Health and Information Sciences, University of Louisville, Louisville, USA

    Qunwei Zhang

Bibliographic Information

  • Book Title: Nanotoxicity

  • Book Subtitle: Methods and Protocols

  • Editors: Qunwei Zhang

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8916-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8915-7Published: 14 December 2018

  • eBook ISBN: 978-1-4939-8916-4Published: 13 December 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 371

  • Number of Illustrations: 16 b/w illustrations, 52 illustrations in colour

  • Topics: Pharmacology/Toxicology

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