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FOXO Transcription Factors

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Wolfgang Link

    1. Instituto de Investigaciones Biomédicas “Alberto Sols” (CSIC-UAM), Madrid, Spain

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1890)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Introduction to FOXO Biology

    • Wolfgang Link
    Pages 1-9

  3. Biochemical and Molecular Methods

    1. Front Matter

      Pages 11-11
      Download chapter PDF

    2. Characterization of MicroRNAs Regulating FOXO Expression

      • Doug Hanniford, Eva Hernando
      Pages 13-28

    3. Identification of Transcription Factor-Binding Sites in the Mouse FOXO1 Promoter

      • Christopher P. Cardozo
      Pages 29-40

    4. Investigating Mechanisms that Control Ubiquitin-Mediated DAF-16/FOXO Protein Turnover

      • Thomas Heimbucher, Coleen T. Murphy
      Pages 41-49

    5. Phosphorylation of FOXO Proteins as a Key Mechanism to Regulate Their Activity

      • Alba Orea-Soufi, David Dávila, María Salazar-Roa, María de Mar Lorente, Guillermo Velasco
      Pages 51-59

    6. Methodological Approach for the Evaluation of FOXO as a Positive Regulator of Antioxidant Genes

      • María Monsalve, Ignacio Prieto, Andreza Fabro de Bem, Yolanda Olmos
      Pages 61-76

    7. Characterization of FOXO Acetylation

      • Shang Yao, Zimam Mahmud, Nikoleta Sachini, Sathid Aimjongjun, Paula Saavedra-García, Eric W-F Lam
      Pages 77-90

    8. Transcriptional Activity of FOXO Transcription Factors Measured by Luciferase Assays

      • Antonia Ávila-Flores, Javier Arranz-Nicolás, Isabel Mérida
      Pages 91-102

    9. Monitoring the Transcriptional Activity of FOXO Transcription Factors by Analyzing their Target Genes

      • Vanessa Henriques, Susana Machado, Wolfgang Link, Bibiana I. Ferreira
      Pages 103-113

    10. Using ChIP-Based Approaches to Characterize FOXO Recruitment to its Target Promoters

      • Neeraj Kumar, Arnab Mukhopadhyay
      Pages 115-130

    11. RNAi-Mediated Silencing of FOXO Factors

      • Priyankar Sanphui, Akash Saha, Subhas Chandra Biswas
      Pages 131-140

  4. Imaging Approaches

    1. Front Matter

      Pages 141-141
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    2. Immunofluorescence Analysis by Confocal Microscopy for Detecting Endogenous FOXO

      • Francisco Castillo, Thomas A. Mackenzie, Bastien Cautain
      Pages 143-149

    3. High-Throughput Image-Based Screening to Identify Chemical Compounds Capable of Activating FOXO

      • Marta Barradas, Wolfgang Link, Diego Megias, Pablo J. Fernandez-Marcos
      Pages 151-161

    4. Image-based Identification of Chemical Compounds Capable of Trapping FOXO in the Cell Nucleus

      • Susana Machado, Catarina Raposo, Bibiana I. Ferreira, Wolfgang Link
      Pages 163-170

    5. Quantifying Tissue-Specific Overexpression of FOXO in Drosophila via mRNA Fluorescence In Situ Hybridization Using Branched DNA Probe Technology

      • Anna C. Blice-Baum, Georg Vogler, Meera C. Viswanathan, Bosco Trinh, Worawan B. Limpitikul, Anthony Cammarato
      Pages 171-190

  5. Omics and Bioinformatic Approaches

    1. Front Matter

      Pages 191-191
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    2. Genome-Wide Analysis for Identifying FOXO Protein-Binding Sites

      • Dong-Ju Shin, Pujan Joshi, Dong-Guk Shin, Li Wang
      Pages 193-203
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Keywords

About this book

This volume provides an overview of experimental procedures and state-of art methods to investigate FOXOs. Chapters guide readers through biochemical and molecular methods, imaging approaches to monitor the subcellular localization of FOXO factors, omics and bioinformatics approaches, different animal models used in FOXO research, and human studies to investigate the role of FOXO factors in human disease and longevity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge FOXO Transcription Factors: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Instituto de Investigaciones Biomédicas “Alberto Sols” (CSIC-UAM), Madrid, Spain

    Wolfgang Link

Bibliographic Information

  • Book Title: FOXO Transcription Factors

  • Book Subtitle: Methods and Protocols

  • Editors: Wolfgang Link

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8900-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8899-0Published: 10 November 2018

  • Softcover ISBN: 978-1-4939-9409-0Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8900-3Published: 09 November 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 270

  • Number of Illustrations: 21 b/w illustrations, 33 illustrations in colour

  • Topics: Protein Science

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