Skip to main content
SpringerLink
Log in
SpringerLink
Log in

Myogenesis

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Sissel Beate Rønning

    1. Nofima AS, Ås, Norway

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1889)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (20 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Purification of Myogenic Progenitors from Human Muscle Using Fluorescence-Activated Cell Sorting (FACS)

    • Anna Pakula, Janelle M. Spinazzola, Emanuela Gussoni
    Pages 1-15

  3. Electrical Pulse Stimulation of Primary Human Skeletal Muscle Cells

    • Nataša Nikolić, Vigdis Aas
    Pages 17-24

  4. Transdifferentiation of Muscle Satellite Cells to Adipose Cells Using CRISPR/Cas9-Mediated Targeting of MyoD

    • Jingjuan Chen, Chao Wang, Shihuan Kuang
    Pages 25-41

  5. Chromatin Immunoprecipitation in Skeletal Myoblasts

    • Vinay Kumar Rao, Shilpa Rani Shankar, Reshma Taneja
    Pages 43-54

  6. Exercising Bioengineered Skeletal Muscle In Vitro: Biopsy to Bioreactor

    • Daniel C. Turner, Andreas M. Kasper, Robert A. Seaborne, Alexander D. Brown, Graeme L. Close, Mark Murphy et al.
    Pages 55-79

  7. Isolation and Purification of Satellite Cells from Young Rats by Percoll Density Gradient Centrifugation

    • Yuji Matsuyoshi, Mariko Akahoshi, Mako Nakamura, Ryuichi Tatsumi, Wataru Mizunoya
    Pages 81-93

  8. Lentivirus-Mediated RNAi in Skeletal Myogenesis

    • Dongwook Kim, Adriana Reyes-Ordoñez, Jie Chen
    Pages 95-110

  9. Adipogenesis from Bovine Precursors

    • Frea Mehta, Ruud Theunissen, Mark J. Post
    Pages 111-125

  10. Transcriptomic Profiling During Myogenesis

    • Alicja Majewska, Tomasz Domoradzki, Katarzyna Grzelkowska-Kowalczyk
    Pages 127-168

  11. Coculture Method to Obtain Endothelial Networks Within Human Tissue-Engineered Skeletal Muscle

    • Dacha Gholobova, Melanie Gerard, Lisanne Terrie, Linda Desender, Janet Shansky, Herman Vandenburgh et al.
    Pages 169-183

  12. Interaction Between Skeletal Muscle Cells and Extracellular Matrix Proteins Using a Serum Free Culture System

    • Danielle E. Dye, Beverley F. Kinnear, Vishal Chaturvedi, Deirdre R. Coombe
    Pages 185-212

  13. LC-MS Analyses of Lipid Species in Skeletal Muscle Cells and Tissue

    • Marta Moreno-Torres, Jesper F. Havelund, Nils J. Faergeman
    Pages 213-228

  14. A siRNA Mediated Screen During C2C12 Myogenesis

    • Alexis Parenté, Luce Pèrié, Laetitia Magnol, Khaled Bouhouche, Véronique Blanquet
    Pages 229-243

  15. Fluorescence-Activated Cell Sorting of Larval Zebrafish Muscle Stem/Progenitor Cells Following Skeletal Muscle Injury

    • Dhanushika Ratnayake, Peter D. Currie
    Pages 245-254

  16. Preparation of Proliferated Bovine Primary Skeletal Muscle Cells for Bottom-Up Proteomics by LC-MSMS Analysis

    • Eva Veiseth-Kent, Vibeke Høst, Mona E. Pedersen
    Pages 255-266

  17. Myogenesis in Drosophila melanogaster: Dissection of Distinct Muscle Types for Molecular Analysis

    • Anton L. Bryantsev, Lizzet Castillo, Sandy T. Oas, Maria B. Chechenova, Tracy E. Dohn, TyAnna L. Lovato
    Pages 267-281

  18. Measuring Both Glucose Uptake and Myosin Heavy Chain Isoform Expression in Single Rat Skeletal Muscle Fibers

    • Mark W. Pataky, Edward B. Arias, Gregory D. Cartee
    Pages 283-300

  19. Myoblast Phosphoproteomics as a Tool to Investigate Global Signaling Events During Myogenesis

    • Fiona K. Jones, Gemma E. Hardman, Samantha Ferries, Claire E. Eyers, Addolorata Pisconti
    Pages 301-317

  20. Preparation and Culturing of Atlantic Salmon Muscle Cells for In Vitro Studies

    • Tone-Kari K. Oestbye, Elisabeth Ytteborg
    Pages 319-330
Back to top

Keywords

About this book

This detailed volume collects many of the common experimental approaches used to study myogenesis. It covers subjects ranging from isolation and purification protocols, manipulation of muscle cells, transcriptomics and proteomics, metabolism and exercise, and tissue engineering. Presented methods involve different species, including human, bovine, Atlantic salmon, rats, mice, larval zebrafish, and Drosophila melanogaster. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Myogenesis: Methods and Protocols aims to serve as an essential part of many laboratory libraries and to assist researchers throughout the world in revealing the unknowns of myogenesis.

Editors and Affiliations

  • Nofima AS, Ås, Norway

    Sissel Beate Rønning

Bibliographic Information

  • Book Title: Myogenesis

  • Book Subtitle: Methods and Protocols

  • Editors: Sissel Beate Rønning

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8897-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8896-9Published: 27 October 2018

  • Softcover ISBN: 978-1-4939-9408-3Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8897-6Published: 26 October 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 351

  • Number of Illustrations: 20 b/w illustrations, 64 illustrations in colour

  • Topics: Cell Biology

Publish with us

Policies and ethics

Back to top

Search

Navigation