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Metalloproteins

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Yilin Hu

    1. Department of Molecular Biology and Biochemistry, University of California-Irvine, Irvine, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1876)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-x
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  2. Metalloproteins

    1. Front Matter

      Pages 1-1
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    2. Nitrogenases

      • Nathaniel S. Sickerman, Yilin Hu, Markus W. Ribbe
      Pages 3-24

    3. Enzymatic Systems with Homology to Nitrogenase: Biosynthesis of Bacteriochlorophyll and Coenzyme F430

      • Jürgen Moser, Gunhild Layer
      Pages 25-35

    4. Carbon Monoxide Dehydrogenases

      • Jae-Hun Jeoung, Berta M. Martins, Holger Dobbek
      Pages 37-54

    5. Molybdenum-Containing Enzymes

      • Dimitri Niks, Russ Hille
      Pages 55-63

    6. Hydrogenases

      • Nathaniel S. Sickerman, Yilin Hu
      Pages 65-88

  3. Genetic and Biochemical Methods

    1. Front Matter

      Pages 89-89
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    2. Genomic Manipulations of the Diazotroph Azotobacter vinelandii

      • Patricia C. Dos Santos
      Pages 91-109

    3. Purification of Nitrogenase Proteins

      • Chi-Chung Lee, Markus W. Ribbe, Yilin Hu
      Pages 111-124

    4. Expression, Purification, and Activity Analysis of Chlorophyllide Oxidoreductase and Ni2+-Sirohydrochlorin a,c-Diamide Reductase

      • Jürgen Moser, Jan Jasper, José Vazquez Ramos, Sven T. Sowa, Gunhild Layer
      Pages 125-140

    5. Reconstitution of Molybdoenzymes with Bis-Molybdopterin Guanine Dinucleotide Cofactors

      • Paul Kaufmann, Chantal Iobbi-Nivol, Silke Leimkühler
      Pages 141-152

  4. Structural and Spectroscopic Methods

    1. Front Matter

      Pages 153-153
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    2. Crystallization of Nitrogenase Proteins

      • Belinda B. Wenke, Renee J. Arias, Thomas Spatzal
      Pages 155-165

    3. X-Ray Crystallography of Carbon Monoxide Dehydrogenases

      • Jae-Hun Jeoung, Berta M. Martins, Holger Dobbek
      Pages 167-178

    4. X-Ray Absorption Spectroscopy of Metalloproteins

      • Limei Zhang
      Pages 179-195

    5. Electron Paramagnetic Resonance Spectroscopy of Metalloproteins

      • Andrew Jasniewski, Yilin Hu, Markus W. Ribbe
      Pages 197-211

    6. Magnetic Circular Dichroism Spectroscopy of Metalloproteins

      • Brian J. Hales
      Pages 213-225

  5. Chemical Methods and Theoretical Calculations

    1. Front Matter

      Pages 227-227
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    2. Chemical Synthesis of an Asymmetric Mimic of the Nitrogenase Active Site

      • Kazuki Tanifuji, Yasuhiro Ohki
      Pages 229-244
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Keywords

About this book

This volume provides an up-to-date, in-depth overview of the methods that have been applied to studying the complex metalloproteins at a molecular level. Chapters cover a wide range of approaches focusing on genetic, biochemical, spectroscopic, chemical methods, and theoretical calculations. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Metalloproteins: Methods and Protocols aims to be useful for anyone who is interested in metalloprotein research and wants to address the unanswered mechanistic and biosynthetic questions of these fascinating enzyme systems.

Editors and Affiliations

  • Department of Molecular Biology and Biochemistry, University of California-Irvine, Irvine, USA

    Yilin Hu

Bibliographic Information

  • Book Title: Metalloproteins

  • Book Subtitle: Methods and Protocols

  • Editors: Yilin Hu

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8864-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8863-1Published: 13 October 2018

  • Softcover ISBN: 978-1-4939-9403-8Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8864-8Published: 13 October 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 275

  • Number of Illustrations: 22 b/w illustrations, 40 illustrations in colour

  • Topics: Protein Science

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