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Somatic Stem Cells

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1842)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
  2. Introduction

    1. Front Matter

      Pages 1-1
    2. An Update on the Therapeutic Potential of Stem Cells

      • Pranela Rameshwar, Caitlyn A. Moore, Niloy N. Shah, Caroline P. Smith
      Pages 3-27
  3. Stem Cell Study in Model Organisms and Human

    1. Front Matter

      Pages 29-29
    2. Isolation and Analysis of Mesenchymal Progenitors of the Adult Hematopoietic Niche

      • Mayra Garcia, Lihong Weng, Xingbin Hu, Ching-Cheng Chen
      Pages 43-54
    3. Identification and Isolation of Mice and Human Hematopoietic Stem Cells

      • Bijender Kumar, Srideshikan Sargur Madabushi
      Pages 55-68
    4. Identification and Characterization of Hair Follicle Stem Cells

      • Xiaoyang Wang, Yizhan Xing, Yuhong Li
      Pages 69-80
    5. Methods of Mesenchymal Stem Cell Homing to the Blood–Brain Barrier

      • Peter Conaty, Lauren S. Sherman, Yahaira Naaldijk, Henning Ulrich, Alexandra Stolzing, Pranela Rameshwar
      Pages 81-91
    6. 3D Bioprinting and Stem Cells

      • Caitlyn A. Moore, Niloy N. Shah, Caroline P. Smith, Pranela Rameshwar
      Pages 93-103
    7. Characterization of Gastrospheres Using 3D Coculture System

      • Carlos Antônio do Nascimento Santos, Radovan Borojevic, Luiz Eurico Nasciutti, Christina M. Maedatakiya
      Pages 105-121
    8. Markers and Methods to Study Adult Midgut Stem Cells

      • Nathan Pinto, Beyoncé Carrington, Catharine Dietrich, Rachit Sinha, Cristopher Aguilar, Tiffany Chen et al.
      Pages 123-137
    9. Quantitative Analysis of Intestinal Stem Cell Dynamics Using Microfabricated Cell Culture Arrays

      • Leigh A. Samsa, Ian A. Williamson, Scott T. Magness
      Pages 139-166
    10. Detection, Labeling, and Culture of Lung Stem and Progenitor Cells

      • Ivan Bertoncello, Gianni Carraro, Jonathan L. McQualter
      Pages 167-181
    11. Isolation, Characterization and Differentiation of Mouse Cardiac Progenitor Cells

      • Santosh Kumar Yadav, Paras Kumar Mishra
      Pages 183-191
    12. Isolating and Characterizing Adipose-Derived Stem Cells

      • Guangpeng Liu, Xi Chen
      Pages 193-201
    13. Enzyme-Free Isolation of Adipose-Derived Mesenchymal Stem Cells

      • Lauren S. Sherman, Alexandra Condé-Green, Vasanth S. Kotamarti, Edward S. Lee, Pranela Rameshwar
      Pages 203-206
    14. Identification and Characterizations of Annulus Fibrosus-Derived Stem Cells

      • Qianping Guo, Pinghui Zhou, Bin Li
      Pages 207-216
    15. Maintenance of Tendon Stem/Progenitor Cells in Culture

      • Jianying Zhang, James H.-C. Wang
      Pages 217-228

About this book

This second edition volume expands on the previous edition with updated discussions on new genetic, molecular, and cellular methods used to study somatic stem cells. The chapters in this book focus on the isolation, classification, purity, and plasticity of these stem cells in a variety of organic tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Somatic Stem Cells: Methods and Protocols, Second Edition is a valuable resource for both novice and experienced molecular biologists, developmental biologists, tissue engineers, and geneticists who are interested in stem cell research and its potentials in regenerative medicine.



Editors and Affiliations

  • Basic Research Laboratory, Stem Cell Regulation and Animal Aging Section, National Cancer Institute, Frederick, USA

    Shree Ram Singh

  • Department of Medicine-Hematology/Oncology, Rutgers New Jersey Medical School, Newark, USA

    Pranela Rameshwar

Bibliographic Information

  • Book Title: Somatic Stem Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Shree Ram Singh, Pranela Rameshwar

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8697-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8696-5Published: 09 September 2018

  • Softcover ISBN: 978-1-4939-9369-7Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8697-2Published: 08 September 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 284

  • Number of Illustrations: 6 b/w illustrations, 52 illustrations in colour

  • Topics: Cell Biology

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access