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Retinal Degeneration

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1834)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xiv
  2. Molecular Genetics and Tools

    1. Front Matter

      Pages 1-1
    2. Identification and Analysis of Genes Associated with Inherited Retinal Diseases

      • Mubeen Khan, Zeinab Fadaie, Stéphanie S. Cornelis, Frans P. M. Cremers, Susanne Roosing
      Pages 3-27
    3. Testing for Known Retinal Degeneration Mutants in Mouse Strains

      • Khalid Rashid, Katharina Dannhausen, Thomas Langmann
      Pages 45-58
    4. CRISPR/Cas9 Gene Editing In Vitro and in Retinal Cells In Vivo

      • Daniela Benati, Valeria Marigo, Alessandra Recchia
      Pages 59-74
    5. Monitoring Surface Reactions by Combined Western Blot-ELISA Analysis

      • Yuchen Lin, Sarah Irmscher, Christine Skerka
      Pages 75-83
  3. Cell/Tissue Culture Models

    1. Front Matter

      Pages 85-85
    2. Advanced Analysis of Photoreceptor Outer Segment Phagocytosis by RPE Cells in Culture

      • Francesca Mazzoni, Yingyu Mao, Silvia C. Finnemann
      Pages 95-108
    3. Porcine RPE/Choroidal Explant Cultures

      • Alexa Klettner, Yoko Miura
      Pages 109-118
    4. Cell Death Analysis in Retinal Cultures

      • Sarah L. Roche, Ana M. Ruiz-Lopez, Thomas G. Cotter
      Pages 143-152
  4. Animal Models

    1. Front Matter

      Pages 165-165
    2. Light Damage Models of Retinal Degeneration

      • Christian Grimm, Charlotte E. Remé
      Pages 167-178
    3. Induction and Readout of Oxygen-Induced Retinopathy

      • Raffael Liegl, Claudia Priglinger, Andreas Ohlmann
      Pages 179-191
    4. Generation and Analysis of Xenopus laevis Models of Retinal Degeneration Using CRISPR/Cas9

      • Joanna M. Feehan, Paloma Stanar, Beatrice M. Tam, Colette Chiu, Orson L. Moritz
      Pages 193-207

About this book

This volume provides key updates on several methods from the first edition as well as including new novel techniques to address the most recent technological developments and their applications in retinal research Chapters guide readers through gene identification approaches, detailed protocols to generate functional retinal pigment epithelium cells, mouse retina and other animal models, fundus imaging and angiography, and cell-based treatment approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Retinal Degeneration: Methods and Protocols, Second Edition aims to ensure successful scientific work in the further study of this vital field.

Editors and Affiliations

  • Institute of Human Genetics, University of Regensburg, Regensburg, Germany

    Bernhard H. F. Weber

  • Department of Ophthalmology, University of Cologne, Cologne, Germany

    Thomas Langmann

Bibliographic Information

  • Book Title: Retinal Degeneration

  • Book Subtitle: Methods and Protocols

  • Editors: Bernhard H. F. Weber, Thomas Langmann

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8669-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8668-2Published: 16 October 2018

  • Softcover ISBN: 978-1-4939-9360-4Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8669-9Published: 15 October 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIV, 417

  • Number of Illustrations: 52 b/w illustrations, 56 illustrations in colour

  • Topics: Molecular Medicine

Buy it now

Buying options

eBook USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 199.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access