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Histone Variants

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Guillermo A. Orsi

    1. Institut Curie, PSL Research University CNRS, UMR3664, Equipe Labellisée Ligue contre le Cancer, Paris, France

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  2. Geneviève Almouzni

    1. Institut Curie, PSL Research University CNRS, UMR3664, Equipe Labellisée Ligue contre le Cancer, Paris, France

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1832)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xii
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  2. Biochemistry

    1. Front Matter

      Pages 1-1
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    2. Methods for Preparing Nucleosomes Containing Histone Variants

      • Tomoya Kujirai, Yasuhiro Arimura, Risa Fujita, Naoki Horikoshi, Shinichi Machida, Hitoshi Kurumizaka
      Pages 3-20

    3. Characterization of Posttranslational Modifications on Histone Variants

      • Francisco Saavedra, Sebastián Marty-Lombardi, Alejandra Loyola
      Pages 21-49

    4. Purification of Histone Variant-Interacting Chaperone Complexes

      • Hideaki Tagami
      Pages 51-60

    5. Detection of Histone Modification Dynamics during the Cell Cycle by MS-Based Proteomics

      • Moritz Carl Völker-Albert, Andreas Schmidt, Teresa K. Barth, Ignasi Forne, Axel Imhof
      Pages 61-74

  3. Epigenomics

    1. Front Matter

      Pages 75-75
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    2. Histone Native Chromatin Immunoprecipitation

      • Alicia Alonso, Emily Bernstein, Dan Hasson
      Pages 77-104

    3. How to Tackle Challenging ChIP-Seq, with Long-Range Cross-Linking, Using ATRX as an Example

      • Julia Truch, Jelena Telenius, Douglas R. Higgs, Richard J. Gibbons
      Pages 105-130

    4. time-ChIP: A Method to Determine Long-Term Locus-Specific Nucleosome Inheritance

      • Wojciech Siwek, Mariluz Gómez-Rodríguez, Daniel Sobral, Ivan R. Corrêa Jr, Lars E. T. Jansen
      Pages 131-158

    5. MINCE-Seq: Mapping In Vivo Nascent Chromatin with EdU and Sequencing

      • Srinivas Ramachandran, Steven Henikoff
      Pages 159-168

    6. RChIP-Seq: Chromatin-Associated RNA Sequencing in Developmentally Staged Mouse Testes

      • Tatiana A. Soboleva, David J. Tremethick
      Pages 169-184

    7. Bioinformatic Analysis of Nucleosome and Histone Variant Positioning

      • Jakub Mieczkowski, Michael Y. Tolstorukov
      Pages 185-203

  4. Microscopy and Cell Biology

    1. Front Matter

      Pages 205-205
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    2. Imaging Newly Synthesized and Old Histone Variant Dynamics Dependent on Chaperones Using the SNAP-Tag System

      • Júlia Torné, Guillermo A. Orsi, Dominique Ray-Gallet, Geneviève Almouzni
      Pages 207-221

    3. Real-Time De Novo Deposition of Centromeric Histone-Associated Proteins Using the Auxin-Inducible Degradation System

      • Sebastian Hoffmann, Daniele Fachinetti
      Pages 223-241

    4. Live Imaging of Parental Histone Variant Dynamics in UVC-Damaged Chromatin

      • Juliette Dabin, Anna Fortuny, Sandra Piquet, Sophie E. Polo
      Pages 243-253

    5. CRISPR/Cas9 Gene Editing of Human Histone H2A Variant H2AX and MacroH2A

      • Justin W. C. Leung, Lara E. Emery, Kyle M. Miller
      Pages 255-269

  5. Evolution, Development, and Diseases

    1. Front Matter

      Pages 271-271
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    2. Studying the Evolution of Histone Variants Using Phylogeny

      • Antoine Molaro, Ines A. Drinnenberg
      Pages 273-291
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Keywords

About this book

This volume compiles methodologies used for detailed studies of histone variants, from their basic properties to their functional roles in chromatin and as vectors of epigenetic information. Its four sections cover experimental approaches to probe the biochemistry of histone variants and variant nucleosomes; their dynamics throughout the cell cycle and at specific genomic locations; their functional impact on chromosome organization and genome stability; and their importance in development, disease and evolution. Written in the highly successful Methods in Molecular Biology series format, chapters include lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, illustrations on the expected results, and tips on troubleshooting and avoiding known pitfalls. This anthology brings together contributions from many of the most highly recognized investigators in this community.

Cutting-edge and thorough, Histone Variants: Methods and Protocols is a valuable resource for anyone interested in exploring the experimental and collaborative possibilities in this evolving field.


Editors and Affiliations

  • Institut Curie, PSL Research University CNRS, UMR3664, Equipe Labellisée Ligue contre le Cancer, Paris, France

    Guillermo A. Orsi, Geneviève Almouzni

Bibliographic Information

  • Book Title: Histone Variants

  • Book Subtitle: Methods and Protocols

  • Editors: Guillermo A. Orsi, Geneviève Almouzni

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8663-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8662-0Published: 03 August 2018

  • Softcover ISBN: 978-1-4939-9358-1Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8663-7Published: 02 August 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 359

  • Number of Illustrations: 19 b/w illustrations, 43 illustrations in colour

  • Topics: Protein Science

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