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Rho GTPases

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Francisco Rivero

    1. The Hull York Medical School, University of Hull, Hull, United Kingdom

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  • Includes cutting-edge methods and protocols for the study of Rho GTPases
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1821)

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xiv
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  2. Computational Modeling and Imaging

    1. Front Matter

      Pages 1-1
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    2. Computational Modeling of the Dynamics of Spatiotemporal Rho GTPase Signaling: A Systematic Review

      • Shabnam Khatibi, Karina Islas Rios, Lan K. Nguyen
      Pages 3-20

    3. Uncovering Bistability in the Rac1/RhoA Signaling Network Through Integrating Computational Modeling and Experimentation

      • Alex von Kriegsheim, Lan K. Nguyen
      Pages 21-36

    4. Modeling Rho GTPase Dynamics Using Boolean Logic

      • Joseph H. R. Hetmanski, Jean-Marc Schwartz, Patrick T. Caswell
      Pages 37-46

    5. EdgeProps: A Computational Platform for Correlative Analysis of Cell Dynamics and Near-Edge Protein Activity

      • Anastasia Zhurikhina, Timothy Qi, Klaus M. Hahn, Timothy C. Elston, Denis Tsygankov
      Pages 47-56

    6. Applying Perturbation Expectation-Maximization to Protein Trajectories of Rho GTPases

      • Peter K. Koo, Simon G. J. Mochrie
      Pages 57-70

    7. Parallel High-Resolution Imaging of Leukocyte Chemotaxis Under Agarose with Rho-Family GTPase Biosensors

      • George R. R. Bell, Dean E. Natwick, Sean R. Collins
      Pages 71-85

    8. Characterization of Genetically Encoded FRET Biosensors for Rho-Family GTPases

      • Sara K. Donnelly, Veronika Miskolci, Alice M. Garrastegui, Dianne Cox, Louis Hodgson
      Pages 87-106

    9. Screening for Rho GTPase Modulators in Actin-Dependent Processes Exemplified by Phagocytosis

      • Daniel Schlam, Sergio Grinstein, Spencer A. Freeman
      Pages 107-127

  3. Biochemistry and High Throughput Methods

    1. Front Matter

      Pages 129-129
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    2. An In Vitro Kinase Assay to Assess Rac1 Phosphorylation by ERK

      • Daniel Brandwein, Junfeng Tong, Laiji Li, Barbara Ballermann, Zhixiang Wang
      Pages 131-140

    3. Methods to Study Rho GTPases Using Bacterial Toxins

      • Gudula Schmidt
      Pages 141-154

    4. Assessment of S-Glutathionylated Rac1 in Cells Using Biotin-Labeled Glutathione

      • Hannah Edenbaum, Jingyan Han
      Pages 155-163

    5. High-Throughput Assay for RhoGEFs Based on the Transcreener® GDP Assay

      • Robert G. Lowery, Meera Kumar
      Pages 165-175

    6. Small-Volume Flow Cytometry-Based Multiplex Analysis of the Activity of Small GTPases

      • Peter Simons, Virginie Bondu, Angela Wandinger-Ness, Tione Buranda
      Pages 177-195Open Access

  4. Cell Types and Processes

    1. Front Matter

      Pages 197-197
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    2. Methods to Study the Roles of Rho GTPases in Platelet Function

      • Francisco Rivero, Simon Calaminus
      Pages 199-217

    3. Methods to Investigate the Role of Rho GTPases in Osteoclast Function

      • Anne Morel, Anne Blangy, Virginie Vives
      Pages 219-233

    4. Methods to Study the Role of Cdc42, Rac1, and Rac2 in B-Cell Cytoskeletal Responses

      • Natalija Gerasimcik, Lisa S. Westerberg, Eva Severinson
      Pages 235-246
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Keywords

About this book

This detailed book expands upon the previous edition with a collection of methods for those performing experimental work on small GTPases of the Rho family. Split into four sections, the volume explores computational modeling and imaging procedures, biochemical methods related to post-translational modifications of Rho GTPases as well as some high throughput methods, functional assays that allow for monitoring the consequences of manipulating Rho GTPases in a variety of cell types and cell biology processes, and techniques specifically designed for studies in selected non-mammalian model organisms (zebrafish, social ameba, plants and algae). Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on trouble shooting and avoiding known pitfalls. 

Authoritative and up-to-date, Rho GTPases: Methods and Protocols, Second Edition constitutes an invaluable tool for all those with an interest in this remarkable family of signaling proteins.

Editors and Affiliations

  • The Hull York Medical School, University of Hull, Hull, United Kingdom

    Francisco Rivero

Bibliographic Information

  • Book Title: Rho GTPases

  • Book Subtitle: Methods and Protocols

  • Editors: Francisco Rivero

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8612-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8611-8Published: 31 July 2018

  • Softcover ISBN: 978-1-4939-9343-7Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8612-5Published: 30 July 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIV, 419

  • Number of Illustrations: 11 b/w illustrations, 29 illustrations in colour

  • Topics: Protein Science

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