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Olfactory Receptors

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Fabio Marques Simoes de Souza

    1. Centro de Matemática Computação e Cognição (CMCC), Universidade Federal do ABC, São Bernardo do Campo, Brazil

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  2. Gabriela Antunes

    1. Centro de Matemática Computação e Cognição (CMCC), Universidade Federal do ABC, São Bernardo do Campo, Brazil

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  • Includes cutting-edge techniques for the study of olfactory receptors
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1820)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
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  2. CD36 Neuronal Identity in the Olfactory Epithelium

    • André Machado Xavier, Isaias Glezer
    Pages 1-19

  3. Deorphanization of Olfactory Trace Amine-Associated Receptors

    • Qian Li
    Pages 21-31

  4. G Protein-Coupled Receptor Kinase 3 (GRK3) in Olfaction

    • Sayoko Ihara, Kazushige Touhara
    Pages 33-41

  5. Virus-Mediated Overexpression of Vomeronasal Receptors and Functional Assessment by Live-Cell Calcium Imaging

    • Pablo Chamero, Frank Zufall
    Pages 43-56

  6. Calcium Imaging of Individual Olfactory Sensory Neurons from Intact Olfactory Turbinates

    • Chunbo Zhang
    Pages 57-68

  7. Fluorescence-Activated Cell Sorting of Olfactory Sensory Neuron Subpopulations

    • Artur G. Leme Silva, Maíra H. Nagai, Bettina Malnic
    Pages 69-76

  8. Numerical Models and In Vitro Assays to Study Odorant Receptors

    • Caroline Bushdid, Claire A. de March, Hiroaki Matsunami, Jérôme Golebiowski
    Pages 77-93

  9. High-Throughput Odorant Receptor Deorphanization Via Phospho-S6 Ribosomal Protein Immunoprecipitation and mRNA Profiling

    • Xiaoyang Serene Hu, Hiroaki Matsunami
    Pages 95-112

  10. Patch-Clamp Recordings from Mouse Olfactory Sensory Neurons

    • Anna Boccaccio
    Pages 113-122

  11. In Vivo Electrophysiological Recordings of Olfactory Receptor Neuron Units and Electro-olfactograms in Anesthetized Rats

    • Patricia Duchamp-Viret, Michel Chaput
    Pages 123-135

  12. Suction Pipette Technique: An Electrophysiological Tool to Study Olfactory Receptor-Dependent Signal Transduction

    • Michele Dibattista, Johannes Reisert
    Pages 137-145

  13. Odor-Induced Electrical and Calcium Signals from Olfactory Sensory Neurons In Situ

    • Xavier Grosmaitre, Minghong Ma
    Pages 147-155

  14. Long-Term Plasticity at the Mitral and Tufted Cell to Granule Cell Synapse of the Olfactory Bulb Investigated with a Custom Multielectrode in Acute Brain Slice Preparations

    • Michael Lukas, Knut Holthoff, Veronica Egger
    Pages 157-167

  15. Multisite Recording of Local Field Potentials in Awake, Free-Moving Mice

    • Xingfeng Mao, Tiantian Cao, Anan Li
    Pages 169-177

  16. In Vivo Two-Photon Imaging of the Olfactory System in Insects

    • Marco Paoli, Albrecht Haase
    Pages 179-219

  17. Approaches for Assessing Olfaction in Children with Autism Spectrum Disorder

    • Hirokazu Kumazaki, Masako Okamoto, Sho Kanzaki, Ken-ichi Okada, Masaru Mimura, Yoshio Minabe et al.
    Pages 221-228

  18. Methods in Rodent Chemosensory Cognition

    • Alan Gelperin
    Pages 229-238

  19. Bioelectronic Nose Using Olfactory Receptor-Embedded Nanodiscs

    • Heehong Yang, Minju Lee, Daesan Kim, Seunghun Hong, Tai Hyun Park
    Pages 239-249

  20. Tracking Odorant Plumes

    • Matthew A. Reidenbach
    Pages 251-263
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Keywords

About this book

This detailed volume explores olfactory receptors, a topic that goes beyond classical biochemical essays and involves methods to investigate the brain areas associated with the perception of odorants. Gathering the strands of many advanced techniques, the book organizes these key methods in order to achieve a unifying comprehension of the olfactory system. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and multidisciplinary, Olfactory Receptors: Methods and Protocols serves to benefit not only those who are beginning in the field of olfactory receptors, but also those who are already working in the field and that want to learn another advanced techniques.

Editors and Affiliations

  • Centro de Matemática Computação e Cognição (CMCC), Universidade Federal do ABC, São Bernardo do Campo, Brazil

    Fabio Marques Simoes de Souza, Gabriela Antunes

Bibliographic Information

  • Book Title: Olfactory Receptors

  • Book Subtitle: Methods and Protocols

  • Editors: Fabio Marques Simoes de Souza, Gabriela Antunes

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8609-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8608-8Published: 08 June 2018

  • Softcover ISBN: 978-1-4939-9342-0Published: 25 July 2019

  • eBook ISBN: 978-1-4939-8609-5Published: 08 June 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 390

  • Number of Illustrations: 19 b/w illustrations, 47 illustrations in colour

  • Topics: Neurosciences

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