Skip to main content
SpringerLink
Log in
Book cover

Receptor-Receptor Interactions in the Central Nervous System

  • Book
  • © 2018

Overview

Editors:
  1. KJELL FUXE

    1. Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Dasiel O. Borroto-Escuela

    1. Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 140)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (21 protocols)

  1. Front Matter

    Pages i-xix
    Download chapter PDF

  2. Analysis and Quantification of GPCR Allosteric Receptor–Receptor Interactions Using Radioligand Binding Assays: The A2AR-D2R Heteroreceptor Complex Example

    • Dasiel O. Borroto-Escuela, Miguel Pérez de la Mora, Michele Zoli, Fabio Benfenati, Manuel Narvaez, Alicia Rivera et al.
    Pages 1-14

  3. Analysis and Quantification of GPCR Heteroreceptor Complexes and Their Allosteric Receptor–Receptor Interactions Using Radioligand Binding Autoradiography

    • Manuel Narvaez, Fidel Corrales, Ismel Brito, Ismael Valladolid-Acebes, Kjell Fuxe, Dasiel O. Borroto-Escuela
    Pages 15-23

  4. On the Study of D4R-MOR Receptor–Receptor Interaction in the Rat Caudate Putamen: Relevance on Morphine Addiction

    • Alicia Rivera, Alejandra Valderrama-Carvajal, Diana Suárez-Boomgaard, Kirill Shumilov, M. Ángeles Real, Kjell Fuxe et al.
    Pages 25-39

  5. Use of Superfused Synaptosomes to Understand the Role of Receptor–Receptor Interactions as Integrative Mechanisms in Nerve Terminals from Selected Brain Region

    • Sarah Beggiato, Sergio Tanganelli, Tiziana Antonelli, Maria Cristina Tomasini, Kjell Fuxe, Dasiel O. Borroto-Escuela et al.
    Pages 41-55

  6. Detection of Fibroblast Growth Factor Receptor 1 (FGFR1) Transactivation by Muscarinic Acetylcholine Receptors (mAChRs) in Primary Neuronal Hippocampal Cultures Through Use of Biochemical and Morphological Approaches

    • Valentina Di Liberto, Giuseppa Mudó, Dasiel O. Borroto-Escuela, Kjell Fuxe, Natale Belluardo
    Pages 57-70

  7. Electrophysiological Approach to GPCR–RTK Interaction Study in Hippocampus of Adult Rats

    • Davide Lattanzi, David Savelli, Michael Di Palma, Stefano Sartini, Silvia Eusebi, Dasiel O. Borroto-Escuela et al.
    Pages 71-90

  8. In Vivo Microdialysis Technique Applications to Understand the Contribution of Receptor–Receptor Interactions to the Central Nervous System Signaling

    • Sergio Tanganelli, Tiziana Antonelli, Sarah Beggiato, Maria Cristina Tomasini, Kjell Fuxe, Dasiel O. Borroto-Escuela et al.
    Pages 91-107

  9. Behavioral Methods to Study the Impact of Receptor–Receptor Interactions in Fear and Anxiety

    • Miguel Pérez de la Mora, José del Carmen Rejón-Orantes, Minerva Crespo-Ramírez, Dasiel O. Borroto-Escuela, Kjell Fuxe
    Pages 109-131

  10. Small Interference RNA Knockdown Rats in Behavioral Functions: GALR1/GALR2 Heteroreceptor in Anxiety and Depression-Like Behavior

    • Antonio Flores-Burgess, Carmelo Millón, Belén Gago, José Angel Narváez, Kjell Fuxe, Zaida Díaz-Cabiale
    Pages 133-148

  11. Double Fluorescent Knock-In Mice to Investigate Endogenous Mu-Delta Opioid Heteromer Subcellular Distribution

    • Lyes Derouiche, Stéphane Ory, Dominique Massotte
    Pages 149-162

  12. Biochemical Characterization of Dopamine D2 Receptor-Associated Protein Complexes Using Co-Immunoprecipitation and Protein Affinity Purification Assays

    • Ping Su, Frankie H. F. Lee, Fang Liu
    Pages 163-186

  13. Methods to Identify the Signature of Trimers Formed by Three G Protein-Coupled Receptors or by Two G Protein-Coupled and One Ionotropic Receptor with Special Emphasis in the Functional Role in the Central Nervous System

    • Irene Reyes-Resina, Eva Martínez-Pinilla, Dasiel O. Borroto-Escuela, Kjell Fuxe, Gemma Navarro, Rafael Franco
    Pages 187-203

  14. Bimolecular Fluorescence Complementation Methodology to Study G Protein-Coupled Receptor Dimerization in Living Cells

    • Doungkamol Alongkronrusmee, Val J. Watts, Richard M. van Rijn
    Pages 205-221

  15. Detection and Quantitative Analysis of Dynamic GPCRs Interactions Using Flow Cytometry-Based FRET

    • Barbara Chruścicka, Shauna E. Wallace Fitzsimons, Clémentine M. Druelle, Timothy G. Dinan, Harriët Schellekens
    Pages 223-238

  16. Assessing GPCR Dimerization in Living Cells: Comparison of the NanoBiT Assay with Related Bioluminescence- and Fluorescence-Based Approaches

    • Elise Wouters, Lakshmi Vasudevan, Francisco Ciruela, Deepak K. Saini, Christophe Stove, Kathleen Van Craenenbroeck
    Pages 239-250

  17. Proximity Biotinylation for Studying G Protein-Coupled Receptor Dimerization

    • Maxwell S. DeNies, Luciana K. Rosselli-Murai, Victoria L. Murray, Elisabeth M. Steel, Allen P. Liu
    Pages 251-263

  18. Conformational Profiling of the 5-HT2A Receptor Using FlAsH BRET

    • Pavel Powlowski, Kyla Bourque, Jace Jones-Tabah, Rory Sleno, Dominic Devost, Terence E. Hébert
    Pages 265-282

  19. Searching the GPCR Heterodimer Network (GPCR-hetnet) Database for Information to Deduce the Receptor–Receptor Interface and Its Role in the Integration of Receptor Heterodimer Functions

    • Ismel Brito, Manuel Narvaez, David Savelli, Kirill Shumilov, Michael Di Palma, Stefano Sartini et al.
    Pages 283-298

  20. Detection, Analysis, and Quantification of GPCR Homo- and Heteroreceptor Complexes in Specific Neuronal Cell Populations Using the In Situ Proximity Ligation Assay

    • Dasiel O. Borroto-Escuela, Manuel Narvaez, Ismael Valladolid-Acebes, Kirill Shumilov, Michael Di Palma, Karolina Wydra et al.
    Pages 299-315
Back to top

Keywords

About this book

This volume covers methodologies, ranging from the molecular level to the network level, used to study receptor-receptor interactions in heteroreceptor complexes inside the central nervous system. The chapters in this book cover topics such as biochemical binding techniques; receptor autoradiography; superfused synaptosome techniques; RTK-GPCR interaction; fluorescence and bioluminiscence energy transfer methods, Co-IP cytometry-based FRET; and novel bioinformatic approaches to understand membrane heteroreceptor complexes and the global panorama of their receptor-receptor interactions. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and thorough, Receptor-Receptor Interactions in the Central Nervous System is a valuable resource for any scientist or researcher interested in this field of study.



Editors and Affiliations

  • Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden

    KJELL FUXE, Dasiel O. Borroto-Escuela

Bibliographic Information

  • Book Title: Receptor-Receptor Interactions in the Central Nervous System

  • Editors: KJELL FUXE, Dasiel O. Borroto-Escuela

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-4939-8576-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8575-3Published: 02 August 2018

  • Softcover ISBN: 978-1-4939-9331-4Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8576-0Published: 01 August 2018

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XIX, 346

  • Number of Illustrations: 17 b/w illustrations, 46 illustrations in colour

  • Topics: Neurosciences

Publish with us

Policies and ethics

Back to top

Search

Navigation