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Molecular Motors

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Christophe Lavelle

    1. National Museum of Natural History, CNRS UMR7196 / INSERM U1154, Paris, France

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1805)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Cellular and Nuclear Forces: An Overview

    • Bidisha Sinha, Arikta Biswas, Gautam V. Soni
    Pages 1-29

  3. Membrane Motors

    1. Front Matter

      Pages 31-31
      Download chapter PDF

    2. The Bacterial Flagellar Rotary Motor in Action

      • Nicolas J. Delalez, Judith P. Armitage
      Pages 33-49

    3. Purification and Reconstitution of Ilyobacter tartaricus ATP Synthase

      • Ganna O. Krasnoselska, Thomas Meier
      Pages 51-71

  4. Cytoskeletal Motors

    1. Front Matter

      Pages 73-73
      Download chapter PDF

    2. Using Microfluidics Single Filament Assay to Study Formin Control of Actin Assembly

      • Guillaume Romet-Lemonne, Bérengère Guichard, Antoine Jégou
      Pages 75-92

    3. Engineering Synthetic Myosin Filaments Using DNA Nanotubes

      • Ruth F. Sommese, Sivaraj Sivaramakrishnan
      Pages 93-101

    4. Direct Imaging of Walking Myosin V by High-Speed Atomic Force Microscopy

      • Noriyuki Kodera, Toshio Ando
      Pages 103-122

    5. High-Resolution Single-Molecule Kinesin Assays at kHz Frame Rates

      • Keith J. Mickolajczyk, William O. Hancock
      Pages 123-138

    6. Multicolor Tracking of Molecular Motors at Nanometer Resolution

      • Sara M. Wichner, Ahmet Yildiz
      Pages 139-149

    7. High-Speed Optical Tweezers for the Study of Single Molecular Motors

      • L. Gardini, A. Tempestini, F. S. Pavone, M. Capitanio
      Pages 151-184

    8. Determining Stable Single Alpha Helical (SAH) Domain Properties by Circular Dichroism and Atomic Force Microscopy

      • Matthew Batchelor, Marcin Wolny, Marta Kurzawa, Lorna Dougan, Peter J. Knight, Michelle Peckham
      Pages 185-211

  5. Nucleic Acids Motors

    1. Front Matter

      Pages 213-213
      Download chapter PDF

    2. The Role of Supercoiling in the Motor Activity of RNA Polymerases

      • Annick Lesne, Jean-Marc Victor, Edouard Bertrand, Eugenia Basyuk, Maria Barbi
      Pages 215-232

    3. Single-Molecule FRET Analysis of Replicative Helicases

      • Seung-Jae Lee, Salman Syed, Taekjip Ha
      Pages 233-250

    4. Recombinases and Related Proteins in the Context of Homologous Recombination Analyzed by Molecular Microscopy

      • Pauline Dupaigne, Eliana Moreira Tavares, Olivier Piétrement, Eric Le Cam
      Pages 251-270

    5. DNA Organization and Superesolved Segregation

      • Diego I. Cattoni, Jean-Bernard Fiche, Antoine Le Gall, Marcelo Nollmann
      Pages 271-289

    6. Electrophoretic Analysis of the DNA Supercoiling Activity of DNA Gyrase

      • Belén Martínez-García, Antonio Valdés, Joana Segura, Silvia Dyson, Ofelia Díaz-Ingelmo, Joaquim Roca
      Pages 291-300

    7. Single-Molecule Angular Optical Trapping for Studying Transcription Under Torsion

      • Jie Ma, Chuang Tan, Michelle D. Wang
      Pages 301-332
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Keywords

About this book

This volume expands on the previous edition with a more extensive look at molecular motors and their roles in muscle contractions, vesicle transport, flagellar beating, chromosome segregation, and DNA replication and repair. The chapters in this book are divided into three parts: Part One looks at membrane motors, such as the bacterial flagellar rotary motor; Part Two discusses cytoskeletal motors, such as kinesin and myosin; and Part Three talks about nucleic acid motors, such as DNA polymerases, helicases, and nucleosome remodelers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and comprehensive, Molecular Motors: Methods and Protocols, Second Edition is a valuable resource for (bio)physicistsand molecular/cellular biologists whose research delves into the mechanisms at work in cells and the motors which power them.

Editors and Affiliations

  • National Museum of Natural History, CNRS UMR7196 / INSERM U1154, Paris, France

    Christophe Lavelle

Bibliographic Information

  • Book Title: Molecular Motors

  • Book Subtitle: Methods and Protocols

  • Editors: Christophe Lavelle

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8556-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8554-8Published: 04 July 2018

  • Softcover ISBN: 978-1-4939-9324-6Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8556-2Published: 03 July 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 452

  • Number of Illustrations: 19 b/w illustrations, 96 illustrations in colour

  • Topics: Cell Biology

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