Skip to main content
  • Book
  • © 2018

Gangliosides

Methods and Protocols

  • Includes cutting-edge techniques for the study of gangliosides
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1804)

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (23 protocols)

  1. Front Matter

    Pages i-xv
  2. Chemical and Physicochemical Properties of Gangliosides

    • Laura Mauri, Sandro Sonnino, Alessandro Prinetti
    Pages 1-17
  3. Gangliosides of the Nervous System

    • Robert Ledeen, Gusheng Wu
    Pages 19-55
  4. Ganglioside Metabolism and Its Inherited Diseases

    • Bernadette Breiden, Konrad Sandhoff
    Pages 97-141
  5. Gangliosides and Tumors

    • Reiji Kannagi, Bi-He Cai, Hsiang-Chi Huang, Chia-Chun Chao, Keiichiro Sakuma
    Pages 143-171
  6. Mass Spectrometry of Gangliosides

    • Akemi Suzuki, Minoru Suzuki, Emi Ito, Takahiro Nitta, Jin-ichi Inokuchi
    Pages 207-221
  7. Immunoelectron Microscopy of Gangliosides

    • Takuma Tsuji, Akikazu Fujita, Toyoshi Fujimoto
    Pages 231-239
  8. Nuclear Magnetic Resonance of Gangliosides

    • Domenico Acquotti, Laura Mauri, Sandro Sonnino
    Pages 241-284
  9. Methods for the Preparation of Anti-ganglioside Monoclonal Antibodies

    • Noriyuki Yuasa, Ikuo Kawashima
    Pages 285-291
  10. Chemical Synthesis of Gangliosides

    • Akihiro Imamura, Makoto Kiso
    Pages 293-310
  11. Radioactive Gangliosides for Biological Studies

    • Laura Mauri, Simona Prioni, Maria Grazia Ciampa, Sandro Sonnino
    Pages 311-322
  12. Synthesis of Fluorescent Gangliosides

    • Günter Schwarzmann
    Pages 323-356
  13. Methods for Assay of Ganglioside Catabolic Enzymes

    • Nicoletta Loberto, Giulia Lunghi, Domitilla Schiumarini, Maura Samarani, Elena Chiricozzi, Massimo Aureli
    Pages 383-400
  14. Pharmacological Modulation of Glycosphingolipid Metabolism

    • Jin-ichi Inokuchi, Takashi Ode, Miki Hara-Yokoyama
    Pages 401-410

About this book

This detailed volume explores methods of examining gangliosides, membrane amphiphilic lipids that are deeply involved in the modulation of the overall properties of cellular membranes, as well as of the functions of membrane-associated proteins. The contents take into account the vital availability of new enzymatic approaches, the knowledge of an incredibly extensive number of glyco-enzymes, and the improvement of multi-genetic analyses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Gangliosides: Methods and Protocols serves as a valuable guidebook for entering in the vast research world of gangliosides.

Editors and Affiliations

  • Department of Medical Biotech & Translational Medicine, University of Milan, Segrate, Italy

    Sandro Sonnino, Alessandro Prinetti

Bibliographic Information

  • Book Title: Gangliosides

  • Book Subtitle: Methods and Protocols

  • Editors: Sandro Sonnino, Alessandro Prinetti

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8552-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8551-7Published: 21 June 2018

  • Softcover ISBN: 978-1-4939-9323-9Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8552-4Published: 20 June 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 456

  • Number of Illustrations: 45 b/w illustrations, 56 illustrations in colour

  • Topics: Biochemistry, general

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access