Immunotoxicity Testing

1. Front Matter

   Pages i-xiii

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2. Overview and Health Risk Considerations

   1. Front Matter

      Pages 1-1

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   2. Immunotoxicology: A Brief History

      • Kenneth L. Hastings

      Pages 3-13

   3. Clinical Immunotoxicology

      • L. Peyton Myers

      Pages 15-26

   4. Investigative Immunotoxicology

      • Stacey E. Anderson, Hillary L. Shane

      Pages 27-46

   5. Developmental Immunotoxicity (DIT) Testing: Current Recommendations and the Future of DIT Testing

      • Annalise N. vonderEmbse, Jamie C. DeWitt

      Pages 47-56

   6. Markers of Inflammation

      • Dori R. Germolec, Kelly A. Shipkowski, Rachel P. Frawley, Ellen Evans

      Pages 57-79

3. Legacy Protocols: In Vivo

   1. Front Matter

      Pages 81-81

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   2. The Sheep Erythrocyte T-Dependent Antibody Response (TDAR)

      • Gregory S. Ladics

      Pages 83-94

   3. Methylated Bovine Serum Albumin (mBSA)-Induced Delayed-Type Hypersensitivity in Mice

      • Jessica Margaret Sido

      Pages 95-99

   4. Use of the LLNA:BrdU-ELISA for Skin Sensitization Hazard Assessment

      • David M. Lehmann

      Pages 101-116

   5. Host Resistance Assays

      • Stefanie C. M. Burleson, Wendy Jo Freebern, Florence G. Burleson, Gary R. Burleson, Victor J. Johnson, Robert W. Luebke

      Pages 117-145

   6. Enhanced Histopathology Evaluation of Lymphoid Organs

      • Susan A. Elmore

      Pages 147-168

   7. Tumor Challenges in Immunotoxicity Testing

      • Sheung P. Ng, Judith T. Zelikoff

      Pages 169-180

4. Legacy Protocols: Ex Vivo and In Vitro

   1. Front Matter

      Pages 181-181

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   2. Flow Cytometry for the Immunotoxicologist

      • Scott T. Espenschied, Robert M. Tighe, Kymberly M. Gowdy

      Pages 183-197

   3. Evaluation of Cell-Mediated Immune Function Using the Cytotoxic T-Lymphocyte Assay

      • Gary R. Burleson, Florence G. Burleson, Rodney R. Dietert

      Pages 199-208

   4. Evaluation of Cell Proliferation and Apoptosis in Immunotoxicity Testing

      • Mitzi Nagarkatti, Sadiye Amcaoglu Rieder, Prakash S. Nagarkatti

      Pages 209-230

   5. Natural Killer (NK) Cell Assays in Immunotoxicity Testing

      • Qing Li

      Pages 231-241

   6. Dendritic Cell Assays

      • Joanna M. Kreitinger, David M. Shepherd

      Pages 243-253

This fully updated volume utilizes the expertise of scientists currently engaged in immunotoxicity testing to provide the reader with lab-ready procedures and the background information needed to identify effective testing approaches. Dedicated to identifying and describing exogenous agents that can modify immune function, uncovering modes and mechanisms of action for such agents, and translating data from the laboratory and from the clinic to better predict health risks as well as benefits to those who are exposed to immunomodulatory agents, immunotoxicity testing continues to be a vital field of study, and this collection highlights both the “tried and true” methods as well as alternative protocols that have been more recently developed.  Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, andtips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Immunotoxicity Testing: Methods and Protocols, Second Edition serves as a valuable contribution to the continued evolution and the application of immunotoxicity testing.

• Immunotoxicology
• Immune function
• Immunomodulatory agents
• Pharmaceuticals
• Health risk reduction
• Xenobiotics

• Department of Pharmacology and Toxicology, East Carolina University, Greenville, USA

  Jamie C. DeWitt

• Department of Pharmacology and Toxicology, Michigan State University, East Lansing, USA

  Cheryl E. Rockwell

• Durham, USA

  Christal C. Bowman

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