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HLA Typing

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1802)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
  2. The Past, Present, and Future of HLA Typing in Transplantation

    • Claire H. Edgerly, Eric T. Weimer
    Pages 1-10
  3. Role of Human Leukocyte Antigens (HLA) in Autoimmune Diseases

    • Gergely Bodis, Victoria Toth, Andreas Schwarting
    Pages 11-29
  4. The IPD Databases: Cataloguing and Understanding Allele Variants

    • Jashan P. Abraham, Dominic J. Barker, James Robinson, Giuseppe Maccari, Steven G. E. Marsh
    Pages 31-48
  5. Allele Frequency Net Database

    • Faviel F. Gonzalez-Galarza, Antony McCabe, Eduardo J. Melo dos Santos, Louise Takeshita, Gurpreet Ghattaoraya, Andrew R. Jones et al.
    Pages 49-62
  6. High-Resolution HLA-Typing by Next-Generation Sequencing of Randomly Fragmented Target DNA

    • Michael Wittig, Simonas Juzenas, Melanie Vollstedt, Andre Franke
    Pages 63-88
  7. Super High Resolution for Single Molecule-Sequence-Based Typing of Classical HLA Loci Using Ion Torrent PGM

    • Takashi Shiina, Shingo Suzuki, Jerzy K. Kulski, Hidetoshi Inoko
    Pages 115-133
  8. Full-Length HLA Class I Genotyping with the MinION Nanopore Sequencer

    • Kathrin Lang, Vineeth Surendranath, Philipp Quenzel, Gerhard Schöfl, Alexander H. Schmidt, Vinzenz Lange
    Pages 155-162
  9. In Silico Typing of Classical and Non-classical HLA Alleles from Standard RNA-Seq Reads

    • Sebastian Boegel, Thomas Bukur, John C. Castle, Ugur Sahin
    Pages 177-191
  10. Comprehensive HLA Typing from a Current Allele Database Using Next-Generation Sequencing Data

    • Shuji Kawaguchi, Koichiro Higasa, Ryo Yamada, Fumihiko Matsuda
    Pages 225-233
  11. AmpliSAS and AmpliHLA: Web Server Tools for MHC Typing of Non-Model Species and Human Using NGS Data

    • Alvaro Sebastian, Magdalena Migalska, Aleksandra Biedrzycka
    Pages 249-273
  12. HLA Haplotype Frequency Estimation from Real-Life Data with the Hapl-o-Mat Software

    • Jürgen Sauter, Christian Schäfer, Alexander H. Schmidt
    Pages 275-284

About this book

This volume explores the rapidly evolving field of HLA typing and its use in both the laboratory setting and in silico methods. The chapters in this book discuss high-throughput methods for HLA typing; wet lab protocols; microarray data and its uses; in silico tools for the identification of HLA alleles from DNA and RNA next-generation-sequencing data, as well as HLA haplotype frequency estimation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, HLA Typing: Methods and Protocols is a valuable resource for any researcher interested in learning more about this developing field.



Editors and Affiliations

  • Johannes Gutenberg University of Mainz, Mainz, Germany

    Sebastian Boegel

Bibliographic Information

  • Book Title: HLA Typing

  • Book Subtitle: Methods and Protocols

  • Editors: Sebastian Boegel

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8546-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8545-6Published: 02 June 2018

  • Softcover ISBN: 978-1-4939-9321-5Published: 25 July 2019

  • eBook ISBN: 978-1-4939-8546-3Published: 01 June 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 287

  • Number of Illustrations: 14 b/w illustrations, 38 illustrations in colour

  • Topics: Immunology

Buy it now

Buying options

eBook USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 199.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access