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Protein Scaffolds

Design, Synthesis, and Applications

  • Book
  • © 2018

Overview

Editors:
  1. Andrew K. Udit

    1. Department of Chemistry, Occidental College, Los Angeles, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1798)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Nanoreactors via Encapsulation of Catalytic Gold Nanoparticles within Cowpea Chlorotic Mottle Virus Protein Cages

    • Aijie Liu, Mark V. de Ruiter, Stan J. Maassen, Jeroen J. L. M. Cornelissen
    Pages 1-9

  3. Encapsulation of Active Enzymes within Bacteriophage P22 Virus-Like Particles

    • Dustin P. Patterson
    Pages 11-24

  4. Encapsulation of Exogenous Proteins in Vault Nanoparticles

    • Meng Wang, Danny Abad, Valerie A. Kickhoefer, Leonard H. Rome, Shaily Mahendra
    Pages 25-37

  5. Enzyme Encapsulation in an Engineered Lumazine Synthase Protein Cage

    • Yusuke Azuma, Donald Hilvert
    Pages 39-55

  6. Metal Ion-Induced Self-Assembly and Packaging of CCMV Nanocapsules

    • Lise Schoonen, Mark B. van Eldijk, Jan C. M. van Hest
    Pages 57-67

  7. Modification of CCMV Nanocages for Enzyme Encapsulation

    • Lise Schoonen, Jan C. M. van Hest
    Pages 69-83

  8. Synthesis of Cross-Linked 2-Aminoethyl Methacrylate in P22 Viral Capsid via Atom-Transfer Radical Polymerization

    • Shefah Qazi
    Pages 85-93

  9. Synthesis of Metal–Organic Frameworks on Tobacco Mosaic Virus Templates

    • Shaobo Li, Jeremiah J. Gassensmith
    Pages 95-108

  10. TMV Disk Scaffolds for Making sub-30 nm Silver Nanorings

    • Serene Bayram, Omar Zahr, Julia Del Re, Amy Szuchmacher Blum
    Pages 109-118

  11. Multivalent Display Using Hybrid Virus Nanoparticles

    • Steven D. Brown
    Pages 119-140

  12. Synthesis of Triangular Silver and Gold Nanoprisms Using Consensus Sequence Tetratricopeptide Repeat Proteins

    • Xi Geng, Tijana Z. Grove
    Pages 141-153

  13. Type III Secretion Filaments as Templates for Metallic Nanostructure Synthesis

    • Anum Azam Glasgow, Danielle Tullman-Ercek
    Pages 155-171

  14. Incorporation of Non-Canonical Amino Acids into Proteins by Global Reassignment of Sense Codons

    • Katharine Y. Fang, Seth A. Lieblich, David A. Tirrell
    Pages 173-186

  15. Modification of Protein Scaffolds via Copper-Catalyzed Azide–Alkyne Cycloaddition

    • Stanislav Presolski
    Pages 187-193

  16. Antibody Modification of p-Aminophenylalanine-Containing Proteins

    • Adel M. ElSohly, Chawita Netirojjanakul, Matthew B. Francis
    Pages 195-201

  17. Cross-Linked Collagen Gels Using Gold Nanoparticles

    • Katarzyna Slowinska
    Pages 203-212

  18. Genipin Cross-Linking of Elastin and Elastin-Based Proteins

    • Lisa D. Muiznieks
    Pages 213-221

  19. Characterization of Self-Assembled Protein Scaffolds from Collagen-Mimetic Peptides

    • Devyani Shukla, Jayeeta Lahiri, Avanish Singh Parmar
    Pages 223-237

  20. Design and Characterization of Fibers and Bionanocomposites Using the Coiled-Coil Domain of Cartilage Oligomeric Matrix Protein

    • Priya Katyal, Jin Kim Montclare
    Pages 239-263
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Keywords

About this book

This volume provides a wide range of methods and protocols detailing various protein structures as platforms for building architectures with targeted application. Chapters guide the readers through exploiting a number of protein scaffolds including virus nanoparticles, elastin and collagen peptides and proteins, and other protein templates for either building materials or presentation of ligands. Site-specific bioconjugation methods, some unique protein architectures, and techniques that exploit peptide amphiphile micelles and assembly of chaperones are also featured. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Protein Scaffolds: Design, Synthesis, andApplications aims to ensure successful results in the study of this vital field.


Editors and Affiliations

  • Department of Chemistry, Occidental College, Los Angeles, USA

    Andrew K. Udit

Bibliographic Information

  • Book Title: Protein Scaffolds

  • Book Subtitle: Design, Synthesis, and Applications

  • Editors: Andrew K. Udit

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7893-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7892-2Published: 05 June 2018

  • Softcover ISBN: 978-1-4939-9317-8Published: 25 July 2019

  • eBook ISBN: 978-1-4939-7893-9Published: 04 June 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 330

  • Number of Illustrations: 24 b/w illustrations, 52 illustrations in colour

  • Topics: Protein Science

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