Skip to main content
SpringerLink
Log in
Book cover

Teratogenicity Testing

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Luís Félix

    1. Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes e Alto Douro (UTAD), Vila Real, Portugal

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols for the study of teratogenicity testing and assessment
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1797)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 249.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (34 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Introduction to Teratology Testing

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. An Overview of Teratology

      • Ana M. Calado, Maria dos Anjos Pires
      Pages 3-32

    3. Teratology Study Guidelines: An Overview

      • Sofia Alves-Pimenta, Luís Félix, Bruno Colaço, Paula A. Oliveira, Carlos Venâncio
      Pages 33-59

    4. Biological Concerns on the Selection of Animal Models for Teratogenic Testing

      • Sofia Alves-Pimenta, Bruno Colaço, Paula A. Oliveira, Carlos Venâncio
      Pages 61-93

  3. In Vitro-Based Teratology Screening

    1. Front Matter

      Pages 95-95
      Download chapter PDF

    2. The Validated Embryonic Stem Cell Test with Murine Embryonic Stem Cells

      • Lauren M. Walker, Darcie L. V. McClelland-Descalzo, Nicole I. zur Nieden
      Pages 97-124

    3. Human Pluripotent Stem Cells to Assess Developmental Toxicity in the Osteogenic Lineage

      • Joseph V. Madrid, Steven R. Sera, Nicole R. L. Sparks, Nicole I. zur Nieden
      Pages 125-145

    4. Chick Embryonic Cardiomyocyte Micromass System for Assessing Developmental Cardiotoxicity of Drugs

      • Wasay Mohiuddin Shaikh Qureshi, Margaret K. Pratten
      Pages 147-163

    5. Flow Cytometry to Evaluate Potential Developmental Toxicants in the Embryonic Stem Cell

      • Valéria Maria Lara, Kelly C. Santos Roballo, André Tadeu Gotardo, Carlos Eduardo Ambrósio
      Pages 165-175

    6. Morphology-Based Whole Embryo Culture for Developmental Toxicity of Drugs

      • Bo Liang, Chi Chiu Wang
      Pages 177-189

    7. Western Blot Methodologies for Analysis of In Vitro Protein Expression Induced by Teratogenic Agents

      • Carlos Martins-Gomes, Amélia M. Silva
      Pages 191-203

    8. Manipulation of MicroRNAs in Cultured Mouse Embryos: Applications for Developmental Toxicology

      • Fuqiang Yuan, Shao-yu Chen
      Pages 205-214

  4. Animal-Based Teratology Methods

    1. Front Matter

      Pages 215-215
      Download chapter PDF

    2. Insights into the Phenotypic and Behavioral Effects of Teratogenic Drugs in Caenorhabditis elegans

      • Caralina Marín de Evsikova
      Pages 217-232

    3. Effect of Teratogens on Development of Drosophila melanogaster

      • Dong Li, Xiaolin Bi
      Pages 233-241

    4. Cellular Responses in Drosophila melanogaster Following Teratogen Exposure

      • Matheus C. Bianchini, José L. R. Portela, Robson L. Puntel, Daiana S. Ávila
      Pages 243-276

    5. Behavioral Teratogenesis in Drosophila melanogaster

      • Monalisa Mishra, Bedanta Kumar Barik
      Pages 277-298

    6. Evaluation of Teratogenicity of Pharmaceuticals Using FETAX

      • Hariz Islas-Flores, Itzayana Pérez-Alvaréz, Leobardo M. Gómez-Oliván
      Pages 299-307

    7. Histological Observation of Teratogenic Phenotypes Induced in Frog Embryo Assays

      • Mark E. Pownall, Margaret S. Saha
      Pages 309-323
Back to top

Keywords

About this book

This detailed book provides a compilation of laboratory techniques and tests to assess the risks to embryo-fetal development from drug exposure during early developmental stages. After an introduction to teratogenicity testing, the contents explore both in vitro and in vivo techniques in the study of the teratogenic and fetotoxic effects of drugs, as well as numerous animal-based teratology methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and comprehensive, Teratogenicity Testing: Methods and Protocols describes methods that integrate a battery of tests that can be performed in cells, organs, tissues, and animal models for evaluating toxicity and/or the safety of compounds in early developmental stages with the goal of estimating, preventing, or minimizing the teratogenic potential of drugs.

Editors and Affiliations

  • Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes e Alto Douro (UTAD), Vila Real, Portugal

    Luís Félix

Bibliographic Information

  • Book Title: Teratogenicity Testing

  • Book Subtitle: Methods and Protocols

  • Editors: Luís Félix

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7883-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7882-3Published: 13 June 2018

  • Softcover ISBN: 978-1-4939-9314-7Published: 25 July 2019

  • eBook ISBN: 978-1-4939-7883-0Published: 12 June 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 614

  • Number of Illustrations: 23 b/w illustrations, 85 illustrations in colour

  • Topics: Pharmacology/Toxicology

Publish with us

Policies and ethics

Back to top

Search

Navigation