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  • © 2018

Tissue Proteomics

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1788)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xii
  2. Depletion of Myofibril-Associated Proteins Using Selective Protein Extraction as a Tool in Cardiac Proteomics

    • Achim Treumann, Pawel Palmowski, Wing Chiu Tong, Julie Taggart, Nick Morrice, G. Nicholas Europe-Finner et al.
    Pages 1-9
  3. HLA Class I and Class II-Induced Intracellular Signaling and Molecular Associations in Primary Human Endothelial Cells

    • Nicole Valenzuela, Nwe Nwe Soe, Fang Li, Xiaohai Zhang, Yi-Ping Jin, Elaine F. Reed
    Pages 23-41
  4. Optimization for Peptide Sample Preparation for Urine Peptidomics

    • Tara K. Sigdel, Carrie D. Nicora, Wei-Jun Qian, Minnie M. Sarwal
    Pages 63-72
  5. Untargeted Screening of Urinary Peptides Using Offline Nano-Liquid Chromatography: MALDI-TOF/TOF Mass Spectrometry

    • François-Ludovic Sauvage, Sébastien Passeron, Pierre Marquet
    Pages 113-121
  6. LC-SRM-Based Targeted Quantification of Urinary Protein Biomarkers

    • Yuqian Gao, Hui Wang, Carrie D. Nicora, Tujin Shi, Richard D. Smith, Tara K. Sigdel et al.
    Pages 145-156
  7. Differential Adipose Tissue Proteomics

    • Kelly J. Shields, Changgong Wu
    Pages 243-250

About this book

This book presents a compilation of methods that detail improved protein and peptide sample preparation and identification. Chapters guide readers through methods for depletion of myofibril-associated proteins, peptide sample preparation in urinary proteomics, purification of targeted proteins from native tissues, fractionation strategies for protein analysis, and GeLC-MS as a sample preparation method for sample preparation for proteomics using minimal amount of tissue. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Tissue Proteomics: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Professor, Department of Surgery Medicine and Pediatrics Director, Kidney-Pancreas Transplant Program, University of California San Francisco, San Francisco, USA

    Minnie M. Sarwal

  • Asst. Professor, Department of Surgery, University of California San Francisco, San Francisco, USA

    Tara K. Sigdel

Bibliographic Information

  • Book Title: Tissue Proteomics

  • Book Subtitle: Methods and Protocols

  • Editors: Minnie M. Sarwal, Tara K. Sigdel

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7854-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7852-6Published: 18 August 2018

  • Softcover ISBN: 978-1-4939-9304-8Published: 10 December 2019

  • eBook ISBN: 978-1-4939-7854-0Published: 17 August 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 320

  • Number of Illustrations: 6 b/w illustrations, 44 illustrations in colour

  • Topics: Protein Science

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access