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  • © 2018

Plant Metabolomics

Methods and Protocols

Editors:

  1. Carla António

    1. Plant Metabolomics Laboratory, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa (ITQB NOVA), Oeiras, Portugal

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  • Includes cutting-edge methods and protocols for the study of plant metabolomics
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1778)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xiv
    PDF

  2. Review Chapters

    1. Front Matter

      Pages 1-1
      PDF

    2. The Importance of Experimental Design, Quality Assurance, and Control in Plant Metabolomics Experiments

      • Marina C. M. Martins, Camila Caldana, Lucia Daniela Wolf, Luis Guilherme Furlan de Abreu
      Pages 3-17

    5. Gas Chromatography–Mass Spectrometry-Based 13C-Labeling Studies in Plant Metabolomics

      • Valéria F. Lima, Leonardo Perez de Souza, Thomas C. R. Williams, Alisdair R. Fernie, Danilo M. Daloso
      Pages 47-58

    6. Carbon Atomic Survey for Identification of Selected Metabolic Fluxes

      • Leonardo Perez de Souza, Alisdair R. Fernie, Takayuki Tohge
      Pages 59-67

  3. Mass Spectrometry-Based Methodology Chapters

    1. Front Matter

      Pages 69-69
      PDF

    3. Quantification of Soluble Sugars and Sugar Alcohols by LC-MS/MS

      • Regina Feil, John Edward Lunn
      Pages 87-100

    4. Untargeted Analysis of Semipolar Compounds by LC-MS and Targeted Analysis of Fatty Acids by GC-MS/GC-FID: From Plant Cultivation to Extract Preparation

      • Camille Bénard, Sébastien Acket, Yannick Rossez, Olivier Fernandez, Thierry Berton, Yves Gibon et al.
      Pages 101-124

    5. Extraction of Plant Lipids for LC-MS-Based Untargeted Plant Lipidomics

      • Thusitha W. T. Rupasinghe, Ute Roessner
      Pages 125-135

    7. Plant Lipidomics Using UPLC-QTOF-MS

      • Yozo Okazaki, Kazuki Saito
      Pages 157-169

    8. Targeted LC-MS Analysis for Plant Secondary Metabolites

      • Takafumi Shimizu, Mutsumi Watanabe, Alisdair R. Fernie, Takayuki Tohge
      Pages 171-181

    9. An UHPLC-MS/MS Method for Target Profiling of Stress-Related Phytohormones

      • Ondřej Novák, Kristýna Floková
      Pages 183-192

    11. Quantification of Sugars and Organic Acids in Biological Matrices Using GC-QqQ-MS

      • Nirupama Samanmalie Jayasinghe, Himasha Mendis, Ute Roessner, Daniel Anthony Dias
      Pages 207-223

    12. Acquisition of Volatile Compounds by Gas Chromatography–Mass Spectrometry (GC-MS)

      • José G. Vallarino, Alexander Erban, Ines Fehrle, Alisdair R. Fernie, Joachim Kopka, Sonia Osorio
      Pages 225-239

    13. Mass Spectrometry Imaging (MSI) for Plant Metabolomics

      • Berin A. Boughton, Dinaiz Thinagaran
      Pages 241-252
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About this book

This detailed book aims to address the need for standardizing key steps in plant metabolomics research that is still considered a critical issue in the field.  The collection unites diverse mass spectrometry (MS)-based protocols, from sample preparation and extraction of plant primary and secondary metabolites (e.g. a range of sugars, lipids, phytohormones and volatile organic compounds) to metabolite analysis, using exclusively highly sensitive MS-based methodologies, followed by frontline bioinformatics and/or mathematical modeling approaches to produce reliable biological data interpretation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Plant Metabolomics: Methods and Protocols serves both new and well-established researchers in the plant metabolomics field in finding helpful guidelines and useful laboratory-based protocols for setting up their routine plant metabolomics experiments.
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Keywords

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Editors and Affiliations

  • Plant Metabolomics Laboratory, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa (ITQB NOVA), Oeiras, Portugal

    Carla António

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Bibliographic Information

  • Book Title: Plant Metabolomics

  • Book Subtitle: Methods and Protocols

  • Editors: Carla António

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7819-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7818-2Published: 15 May 2018

  • Softcover ISBN: 978-1-4939-9294-2Published: 10 December 2019

  • eBook ISBN: 978-1-4939-7819-9Published: 14 May 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 355

  • Number of Illustrations: 27 b/w illustrations, 51 illustrations in colour

  • Topics: Plant Sciences

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Buy it now

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

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