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Virus-Derived Nanoparticles for Advanced Technologies

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1776)

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Table of contents (41 protocols)

  1. Front Matter

    Pages i-xv
  2. Preparing Virus and Virus-Derived Nanoarchitectures as Containers, Carrier Scaffolds, and Structure-Directing Agents: From Particles to Functional Super-Assemblies

    1. Front Matter

      Pages 1-1
    2. Fabrication of Tobacco Mosaic Virus-Like Nanorods for Peptide Display

      • Emily J. Larkin, Adam D. Brown, James N. Culver
      Pages 51-60
    3. In Planta Production of Fluorescent Filamentous Plant Virus-Based Nanoparticles

      • Sourabh Shukla, Christina Dickmeis, Rainer Fischer, Ulrich Commandeur, Nicole F. Steinmetz
      Pages 61-84
    4. Recombinant Expression of Tandem-HBc Virus-Like Particles (VLPs)

      • Sam L. Stephen, Lucy Beales, Hadrien Peyret, Amy Roe, Nicola J. Stonehouse, David J. Rowlands
      Pages 97-123
    5. Production and Application of Insect Virus-Based VLPs

      • Radhika Gopal, Anette Schneemann
      Pages 125-141
    6. Nanomanufacture of Free-Standing, Porous, Janus-Type Films of Polymer–Plant Virus Nanoparticle Arrays

      • Brylee David B. Tiu, Rigoberto C. Advincula, Nicole F. Steinmetz
      Pages 143-157
  3. Functional Compounds in Virus-Based Containers: From Preparation to Applications of Hybrid Nanoobjects

    1. Front Matter

      Pages 187-187
    2. Internal Deposition of Cobalt Metal and Iron Oxide Within CPMV eVLPs

      • Alaa A. A. Aljabali, David J. Evans
      Pages 189-201
    3. Plant Virus-Based Nanoparticles for the Delivery of Agronomic Compounds as a Suspension Concentrate

      • Richard H. Guenther, Steven A. Lommel, Charles H. Opperman, Tim L. Sit
      Pages 203-214
    4. CCMV-Based Enzymatic Nanoreactors

      • Mark V. de Ruiter, Rindia M. Putri, Jeroen J. L. M. Cornelissen
      Pages 237-247

About this book

This volume details protocols on virus-derived nanoparticles (VNPs) for a number of different applications. Chapters guide readers through the production of  VNPs derived from plant, animal and bacterial viruses, prokaryotic and eukaryotic expression systems,  encapsulation of heterologous materials within VNPs, and the modification of the outer surface of VNPs and how such modified VNPs can be developed into functional entities. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and practical, Virus-Derived Nanoparticles for Advanced Technologies: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Institute of Biomaterials and Biomolecular Systems, University of Stuttgart, Stuttgart, Germany

    Christina Wege

  • Department of Biological Chemistry, John Innes Centre, Norwich, United Kingdom

    George P. Lomonossoff

Bibliographic Information

  • Book Title: Virus-Derived Nanoparticles for Advanced Technologies

  • Book Subtitle: Methods and Protocols

  • Editors: Christina Wege, George P. Lomonossoff

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7808-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7806-9Published: 05 June 2018

  • Softcover ISBN: 978-1-4939-9291-1Published: 25 July 2019

  • eBook ISBN: 978-1-4939-7808-3Published: 04 June 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 671

  • Number of Illustrations: 48 b/w illustrations, 108 illustrations in colour

  • Topics: Biotechnology

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access