Intravital Imaging of Dynamic Bone and Immune Systems

1. Front Matter

   Pages i-xi

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2. Bone Imaging: Osteoclast and Osteoblast Dynamics

   • Junichi Kikuta, Masaru Ishii

   Pages 1-9

3. Intravital Imaging of Mouse Bone Marrow: Hemodynamics and Vascular Permeability

   • Yookyung Jung, Joel A. Spencer, Anthony P. Raphael, Juwell W. Wu, Clemens Alt, Judith R. Runnels et al.

   Pages 11-22

4. Bone Imaging: Platelet Formation Dynamics

   • Asuka Sakata, Satoshi Nishimura

   Pages 23-28

5. Live Imaging of Interstitial T Cell Migration Using Lymph Node Slices

   • Tomoya Katakai

   Pages 29-42

6. Two-Photon Imaging of T-Cell Motility in Lymph Nodes: In Vivo and Ex Vivo Approaches

   • Akira Takeda, Masayuki Miyasaka, Eiji Umemoto

   Pages 43-52

7. Imaging the Lymph Node Stroma

   • Clément Ghigo, Rebecca Gentek, Marc Bajénoff

   Pages 53-61

8. Intravital Imaging of B Cell Responses in Lymph Nodes

   • Stefano Sammicheli, Mirela Kuka, Matteo Iannacone

   Pages 63-74

9. Live Imaging of the Skin Immune Responses: Visualization of the Contact Hypersensitivity Response

   • Gyohei Egawa, Tetsuya Honda, Kenji Kabashima

   Pages 75-85

10. Imaging of Inflammatory Responses in the Mouse Ear Skin

    • Jackson LiangYao Li, Chi Ching Goh, Lai Guan Ng

    Pages 87-107

11. In Vivo Imaging of Immune Cells in Peyer’s Patches

    • Andrea Reboldi

    Pages 109-118

12. Intravital Imaging of T Cells Within the Spinal Cord

    • Naoto Kawakami

    Pages 119-127

13. Kidney Imaging: Intravital Microscopy

    • Takashi Hato, Seth Winfree, Pierre C. Dagher

    Pages 129-136

14. Intravital Imaging of Liver Cell Dynamics

    • Sayaka Matsumoto, Junichi Kikuta, Masaru Ishii

    Pages 137-143

15. Intravital Imaging of the Heart at the Cellular Level Using Two-Photon Microscopy

    • Ryohei Matsuura, Shigeru Miyagawa, Junichi Kikuta, Masaru Ishii, Yoshiki Sawa

    Pages 145-151

16. Imaging Window Device for Subcutaneous Implantation Tumor

    • Wataru Ikeda, Ken Sasai, Tsuyoshi Akagi

    Pages 153-163

17. New Tools for Imaging of Immune Systems: Visualization of Cell Cycle, Cell Death, and Cell Movement by Using the Mice Lines Expressing Fucci, SCAT3.1, and Kaede and KikGR

    • Michio Tomura

    Pages 165-174

18. Back Matter

    Pages 175-175

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This detailed volume presents a number of chapters that will aid researchers in jumping the hurdle preventing the popularization of advanced intravital imaging technology usage, namely the complicated experimental protocols. Leading researchers undertaking imaging studies in the field of bone and immune systems have contributed the chapters that aim to guide readers through these complex methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Intravital Imaging of Dynamic Bone and Immune Systems: Methods and Protocols will serve as an idea guide for general readers in order to establish and perform these experiments by their own. 

• Living cells
• Cellular dynamic movement
• Osteoclasts
• Bone cells
• Multi-photon excitation fluorescent microscopy

• Department of Immunology and Cell Biology, Graduate School of Medicine, Osaka University, Osaka, Japan

  Masaru Ishii

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