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Mouse Retinal Phenotyping

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Naoyuki Tanimoto

    1. Department of Ophthalmology, University Hospital Schleswig-Holstein, Kiel, Germany

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1753)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Overview

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Morphological Survey from Neurons to Circuits of the Mouse Retina

      • Yoshihiko Tsukamoto
      Pages 3-25

    3. Measuring Retinal Function in the Mouse

      • Jan Kremers, Naoyuki Tanimoto
      Pages 27-40

    4. Modeling Retinal Diseases Using Genetic Approaches in Mice

      • Akiko Maeda, Tadao Maeda
      Pages 41-59

  3. Outer to Mid-Retina

    1. Front Matter

      Pages 61-61
      Download chapter PDF

    2. Cell Culture Analysis of the Phagocytosis of Photoreceptor Outer Segments by Primary Mouse RPE Cells

      • Roni A. Hazim, David S. Williams
      Pages 63-71

    3. Two-Photon Microscopy (TPM) and Fluorescence Lifetime Imaging Microscopy (FLIM) of Retinal Pigment Epithelium (RPE) of Mice In Vivo

      • Yoko Miura
      Pages 73-88

    4. RPE Visual Cycle and Biochemical Phenotypes of Mutant Mouse Models

      • Bhubanananda Sahu, Akiko Maeda
      Pages 89-102

    5. Use of Direct Current Electroretinography for Analysis of Retinal Pigment Epithelium Function in Mouse Models

      • Minzhong Yu, Neal S. Peachey
      Pages 103-113

    6. Disruption of Rhodopsin Dimerization in Mouse Rod Photoreceptors by Synthetic Peptides Targeting Dimer Interface

      • Sandeep Kumar, Alyssia Lambert, Jon Rainier, Yingbin Fu
      Pages 115-128

    7. Experimental Approaches for Defining the Role of the Ca2+-Modulated ROS-GC System in Retinal Rods of Mouse

      • Clint L. Makino, Teresa Duda, Alexandre Pertzev, Rameshwar K. Sharma
      Pages 129-158

    8. Microglia Analysis in Retinal Degeneration Mouse Models

      • Katharina Dannhausen, Khalid Rashid, Thomas Langmann
      Pages 159-166

    9. Determination of Mitochondrial Oxygen Consumption in the Retina Ex Vivo: Applications for Retinal Disease

      • Yogita K. Adlakha, Anand Swaroop
      Pages 167-177

    10. Analysis of Feedback Signaling from Horizontal Cells to Photoreceptors in Mice

      • Arlene A. Hirano, Xue Liu, Nicholas C. Brecha, Steven Barnes
      Pages 179-189

    11. Assessment of the Absolute Excitatory Level of the Retina by Flicker ERG

      • Naoyuki Tanimoto, Mathias W. Seeliger
      Pages 191-202

    12. Ex Vivo Functional Evaluation of Synaptic Transmission from Rods to Rod Bipolar Cells in Mice

      • Johan Pahlberg, Anurima Majumder, Nikolai O. Artemyev
      Pages 203-216

    13. Functional and Morphological Analysis of OFF Bipolar Cells

      • Chase B. Hellmer, Tomomi Ichinose
      Pages 217-233

  4. Mid- To Inner Retina

    1. Front Matter

      Pages 235-235
      Download chapter PDF

    2. Immunohistochemical Phenotyping of Mouse Amacrine Cell Subtypes

      • Denize Atan
      Pages 237-248
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Keywords

About this book

The aim of this volume is to provide a wide range of techniques and aspects of mouse retinal phenotyping and characterization. Chapters guide readers through morphological and functional properties of the mouse retina and techniques for the analysis of outer to mid-retina and mid- to inner retina. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Mouse Retinal Phenotyping: Methods and Protocols aims to ensure successful results in the further study of this vital field. 

Editors and Affiliations

  • Department of Ophthalmology, University Hospital Schleswig-Holstein, Kiel, Germany

    Naoyuki Tanimoto

Bibliographic Information

  • Book Title: Mouse Retinal Phenotyping

  • Book Subtitle: Methods and Protocols

  • Editors: Naoyuki Tanimoto

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7720-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7719-2Published: 22 March 2018

  • Softcover ISBN: 978-1-4939-9267-6Published: 10 December 2019

  • eBook ISBN: 978-1-4939-7720-8Published: 21 March 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 334

  • Number of Illustrations: 23 b/w illustrations, 52 illustrations in colour

  • Topics: Molecular Medicine

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