Overview
- Editors:
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Marco G. Alves
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Department of Microscopy, Laboratory of Cell Biology and Unit for Multidisciplinary Research in Biomedicine (UMIB), Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Porto, Portugal
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Pedro F. Oliveira
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Department of Microscopy, Laboratory of Cell Biology and Unit for Multidisciplinary Research in Biomedicine (UMIB), Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Porto, Portugal
- Includes cutting-edge techniques for the study and use of Sertoli cells
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
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Table of contents (20 protocols)
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Front Matter
Pages i-xiii
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- Raquel L. Bernardino, Marco G. Alves, Pedro F. Oliveira
Pages 1-8
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- Raquel L. Bernardino, Marco G. Alves, Pedro F. Oliveira
Pages 9-15
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- Barbara Bilinska, Anna Hejmej, Malgorzata Kotula-Balak
Pages 17-36
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- Valeria Grieco, Barbara Banco
Pages 37-47
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- Jesús Martínez-Hernández, Vicente Seco-Rovira, Ester Beltrán-Frutos, Victor Quesada-Cubo, Concepción Ferrer, Luis Miguel Pastor
Pages 49-60
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- Maria João Freitas, Margarida Fardilha
Pages 61-71
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- Maria João Freitas, Margarida Fardilha
Pages 73-84
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- Marina G. Yefimova, Nadia Messaddeq, Annie-Claire Meunier, Anne Cantereau, Bernard Jegou, Nicolas Bourmeyster
Pages 85-101
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- Nabil Eid, Yuko Ito, Akio Horibe, Hitomi Hamaoka, Yoichi Kondo
Pages 103-112
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- Chao Liu, Jehangir Khan, Wei Li
Pages 113-128
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- Luís Crisóstomo, Marco G. Alves, Agostina Gorga, Mário Sousa, María F. Riera, María N. Galardo et al.
Pages 129-155
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- Ivana Jarak, Pedro F. Oliveira, Gustavo Rindone, Rui A. Carvalho, María N. Galardo, María F. Riera et al.
Pages 157-171
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- Rita Ferreira, Fábio Trindade, Rui Vitorino
Pages 173-190
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- Hong Wang, Qingqing Yuan, Minghui Niu, Liping Wen, Hongyong Fu, Fan Zhou et al.
Pages 191-202
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- Diane Rebourcet, Annalucia Darbey, Michael Curley, Peter O’Shaughnessy, Lee B. Smith
Pages 203-228
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- Qing Wen, Elizabeth I. Tang, Nan Li, Dolores D. Mruk, Will M. Lee, Bruno Silvestrini et al.
Pages 229-243
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- Haiqi Chen, Wing-yee Lui, Dolores D. Mruk, Xiang Xiao, Renshan Ge, Qingquan Lian et al.
Pages 245-252
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- Pranitha Jenardhanan, Manivel Panneerselvam, Premendu P. Mathur
Pages 253-277
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- Anna Maggio, Raquel L. Bernardino, Patrizia Gena, Marco G. Alves, Pedro F. Oliveira, Giuseppe Calamita
Pages 279-286
About this book
This detailed volume explores the use of Sertoli cells as models in male reproductive biology or as supporters for other cell types, and it answers the need for standardized protocols for their in vitro culture. Prepared for non-specialists as well as experienced researchers that may be interested in a multidisciplinary approach to study these cells, the book examines these remarkable cells that present some unique features that go far beyond the physical and nutritional support of spermatogenesis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Sertoli Cells: Methods and Protocols serves as a valuable resource for experienced researchers in reproductive biology, particularly andrologists, but also for future investigators and young students starting to study these extraordinary cells.
Reviews
“The pictures illustrating the book are all of high quality and the readers will be satisfied and certainly not disappointed while running through the chapters: exactly in the same manner you cannot stop of eating chapters or cherries, one is calling the other!” (CarloAlberto Redi, European Journal of Histochemistry, Vol. 63 (01), 2019)
