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  • © 2018

Antibiotic Resistance Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1736)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-x
  2. Methods for Measuring the Production of Quorum Sensing Signal Molecules

    • Manuel Alcalde-Rico, José Luis Martínez
    Pages 1-15
  3. Construction and Use of Staphylococcus aureus Strains to Study Within-Host Infection Dynamics

    • Gareth McVicker, Tomasz K. Prajsnar, Simon J. Foster
    Pages 17-27
  4. Detecting Phenotypically Resistant Mycobacterium tuberculosis Using Wavelength Modulated Raman Spectroscopy

    • Vincent O. Baron, Mingzhou Chen, Simon O. Clark, Ann Williams, Kishan Dholakia, Stephen H. Gillespie
    Pages 41-50
  5. A Flow Cytometry Method for Assessing M. tuberculosis Responses to Antibiotics

    • Charlotte L. Hendon-Dunn, Stephen R. Thomas, Stephen C. Taylor, Joanna Bacon
    Pages 51-57
  6. Application of Continuous Culture for Assessing Antibiotic Activity Against Mycobacterium tuberculosis

    • Charlotte L. Hendon-Dunn, Saba Anwar, Christopher Burton, Joanna Bacon
    Pages 59-73
  7. Real-Time Digital Bright Field Technology for Rapid Antibiotic Susceptibility Testing

    • Chiara Canali, Erik Spillum, Martin Valvik, Niels Agersnap, Tom Olesen
    Pages 75-84
  8. Enhanced Methodologies for Detecting Phenotypic Resistance in Mycobacteria

    • Robert J. H. Hammond, Vincent O. Baron, Sam Lipworth, Stephen H. Gillespie
    Pages 85-94
  9. Selection of ESBL-Producing E. coli in a Mouse Intestinal Colonization Model

    • Frederik Boëtius Hertz, Karen Leth Nielsen, Niels Frimodt-Møller
    Pages 105-115
  10. Transcriptional Profiling Mycobacterium tuberculosis from Patient Sputa

    • Leticia Muraro Wildner, Katherine A. Gould, Simon J. Waddell
    Pages 117-128
  11. Use of Larval Zebrafish Model to Study Within-Host Infection Dynamics

    • Tomasz K. Prajsnar, Gareth McVicker, Alexander Williams, Stephen A. Renshaw, Simon J. Foster
    Pages 147-156
  12. Back Matter

    Pages 167-168

About this book

This third edition provides a wide range of different technologies, ranging from conventional growth basic techniques, application of molecular biology, development of resistance mutations, and diagnosis and monitoring treatment response.  New and updated chapters cover techniques from the microscopic scale to whole animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Antibiotic Resistance Protocols, Third Edition aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • School of Medicine, University of St. Andrews, North Haugh, United Kingdom

    Stephen H. Gillespie

Bibliographic Information

  • Book Title: Antibiotic Resistance Protocols

  • Editors: Stephen H. Gillespie

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7638-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2018

  • Hardcover ISBN: 978-1-4939-7636-2Published: 11 January 2018

  • Softcover ISBN: 978-1-4939-8534-0Published: 09 June 2019

  • eBook ISBN: 978-1-4939-7638-6Published: 10 January 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: X, 168

  • Number of Illustrations: 27 b/w illustrations, 5 illustrations in colour

  • Topics: Microbiology

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 159.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access