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Noncanonical Amino Acids

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Edward A. Lemke

    1. Structural and Computational Biology Unit & Cell Biology and Biophysics Unit, EMBL, Heidelberg, Germany

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1728)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Engineering in the Test Tube

    1. Front Matter

      Pages 1-1
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    2. Leveraging Formylglycine-Generating Enzyme for Production of Site-Specifically Modified Bioconjugates

      • Robyn M. Barfield, David Rabuka
      Pages 3-16

    3. Artificial Division of Codon Boxes for Expansion of the Amino Acid Repertoire of Ribosomal Polypeptide Synthesis

      • Yoshihiko Iwane, Takayuki Katoh, Yuki Goto, Hiroaki Suga
      Pages 17-47

    4. Cell-Free Protein Synthesis for Multiple Site-Specific Incorporation of Noncanonical Amino Acids Using Cell Extracts from RF-1 Deletion E. coli Strains

      • Eiko Seki, Tatsuo Yanagisawa, Shigeyuki Yokoyama
      Pages 49-65

    5. Tub-Tag Labeling; Chemoenzymatic Incorporation of Unnatural Amino Acids

      • Jonas Helma, Heinrich Leonhardt, Christian P. R. Hackenberger, Dominik Schumacher
      Pages 67-93

  3. Engineering in Prokaryotes

    1. Front Matter

      Pages 95-95
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    2. Directed Evolution of Orthogonal Pyrrolysyl-tRNA Synthetases in Escherichia coli for the Genetic Encoding of Noncanonical Amino Acids

      • Moritz J. Schmidt, Daniel Summerer
      Pages 97-111

    3. Genetic Code Expansion in Enteric Bacterial Pathogens

      • Huangtao Zheng, Shixian Lin, Peng R. Chen
      Pages 113-126

    4. Self-Directed in Cell Production of Methionine Analogue Azidohomoalanine by Synthetic Metabolism and Its Incorporation into Model Proteins

      • Ying Ma, Martino L. Di Salvo, Nediljko Budisa
      Pages 127-135

    5. Residue-Specific Incorporation of Noncanonical Amino Acids for Protein Engineering

      • Mark B. van Eldijk, Jan C. M. van Hest
      Pages 137-145

    6. Using Amber and Ochre Nonsense Codons to Code Two Different Noncanonical Amino Acids in One Protein Gene

      • Jeffery M. Tharp, Wenshe R. Liu
      Pages 147-154

    7. Genetic Incorporation of Unnatural Amino Acids into Proteins of Interest in Streptomyces venezuelae ATCC 15439

      • Jingxuan He, Charles E. Melançon III
      Pages 155-168

    8. Expression and Purification of Site-Specifically Lysine-Acetylated and Natively-Folded Proteins for Biophysical Investigations

      • Michael Lammers
      Pages 169-190

    9. Site-Specific Incorporation of Sulfotyrosine Using an Expanded Genetic Code

      • Xiang Li, Chang C. Liu
      Pages 191-200

    10. Site-Specific Protein Labeling with Tetrazine Amino Acids

      • Robert J. Blizzard, True E. Gibson, Ryan A. Mehl
      Pages 201-217

  4. Engineering in Eukaryotes

    1. Front Matter

      Pages 219-219
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    2. Mapping of Protein Interfaces in Live Cells Using Genetically Encoded Crosslinkers

      • Lisa Seidel, Irene Coin
      Pages 221-235

    3. Generation of Stable Amber Suppression Cell Lines

      • Simon J. Elsässer
      Pages 237-245

    4. Trapping Chromatin Interacting Proteins with Genetically Encoded, UV-Activatable Crosslinkers In Vivo

      • Christian Hoffmann, Heinz Neumann, Petra Neumann-Staubitz
      Pages 247-262
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Keywords

About this book

This volume covers some of the most widely used protocols on nanocanonical amino acids, providing details and advice for users to get each method up and running for their chosen application.  Chapters have been divided into three parts describing methods for protein production in the test tube, in prokaryotes, and in eukaryotes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Noncanonical Amino Acids: Methods and Protocols aims to provide readers with techniques that enable them to design new experiments and create new areas of research.

Editors and Affiliations

  • Structural and Computational Biology Unit & Cell Biology and Biophysics Unit, EMBL, Heidelberg, Germany

    Edward A. Lemke

Bibliographic Information

  • Book Title: Noncanonical Amino Acids

  • Book Subtitle: Methods and Protocols

  • Editors: Edward A. Lemke

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7574-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2018

  • Hardcover ISBN: 978-1-4939-7573-0Published: 06 February 2018

  • Softcover ISBN: 978-1-4939-8525-8Published: 04 June 2019

  • eBook ISBN: 978-1-4939-7574-7Published: 05 February 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 411

  • Number of Illustrations: 37 b/w illustrations, 39 illustrations in colour

  • Topics: Biochemistry, general

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