Inflammation and Cancer

1. Front Matter

   Pages i-xii

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2. Experimental Model Systems

   1. Front Matter

      Pages 1-1

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   2. In Vivo Models of Inflammatory Bowel Disease and Colitis-Associated Cancer

      • Ka Yee Fung, Tracy Putoczki

      Pages 3-13

   3. Combining Cell Type-Restricted Adenoviral Targeting with Immunostaining and Flow Cytometry to Identify Cells-of-Origin of Lung Cancer

      • Sarah A. Best, Ariena Kersbergen, Marie-Liesse Asselin-Labat, Kate D. Sutherland

      Pages 15-29

   4. Utility of Endoscopic Ultrasound-Guided Fine-Needle Aspiration for Preclinical Evaluation of Therapies in Cancer

      • William Berry, Daniel Croagh

      Pages 31-40

   5. Modelling Intestinal Carcinogenesis Using In Vitro Organoid Cultures

      • Thierry Jardé, Genevieve Kerr, Reyhan Akhtar, Helen E. Abud

      Pages 41-52

   6. Protocols to Evaluate Cigarette Smoke-Induced Lung Inflammation and Pathology in Mice

      • Ross Vlahos, Steven Bozinovski

      Pages 53-63

   7. Tracking Competent Host Defence to Chronic Inflammation: An In Vivo Model of Peritonitis

      • Javier Uceda Fernandez, David Millrine, Simon A. Jones

      Pages 65-75

   8. Citrobacter rodentium Infection Model for the Analysis of Bacterial Pathogenesis and Mucosal Immunology

      • Catherine L. Kennedy, Elizabeth L. Hartland

      Pages 77-89

   9. In Vivo Infection Model of Severe Influenza A Virus

      • Ashley Mansell, Michelle D. Tate

      Pages 91-99

   10. In Vivo Models for Inflammatory Arthritis

       • Gareth W. Jones, David G. Hill, Katie Sime, Anwen S. Williams

       Pages 101-118

   11. Isolation of Mouse Primary Gastric Epithelial Cells to Investigate the Mechanisms of Helicobacter pylori Associated Disease

       • Le Son Tran, Richard L. Ferrero

       Pages 119-126

   12. Dissecting Interleukin-6 Classic- and Trans-Signaling in Inflammation and Cancer

       • Christoph Garbers, Stefan Rose-John

       Pages 127-140

3. Experimental Techniques

   1. Front Matter

      Pages 141-141

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   2. Laser Microdissection of Cellular Compartments for Expression Analyses in Cancer Models

      • Tae-Su Han, Masanobu Oshima

      Pages 143-153

   3. Tissue Processing for Stereological Analyses of Lung Structure in Chronic Obstructive Pulmonary Disease

      • Mohamed Saad, Saleela M. Ruwanpura

      Pages 155-162

   4. Quantifying Caspase-1 Activity in Murine Macrophages

      • Dave Boucher, Amy Chan, Connie Ross, Kate Schroder

      Pages 163-176

   5. Analysis of Histone Modifications in Acute Myeloid Leukaemia Using Chromatin Immunoprecipitation

      • Benjamin J. Shields, Andrew Keniry, Marnie E. Blewitt, Matthew P. McCormack

      Pages 177-184

   6. Analysis of DNA Methylation in Tissues Exposed to Inflammation

      • Naoko Hattori, Toshikazu Ushijima

      Pages 185-199

   7. A Comprehensive Protocol Resource for Performing Pooled shRNA and CRISPR Screens

      • Leonie A. Cluse, Iva Nikolic, Deborah Knight, Piyush B. Madhamshettiwar, Jennii Luu, Karla J. Cowley et al.

      Pages 201-227

This volume provides a broad spectrum of research models, techniques, and protocols. Chapters are divided into sections providing detailed information on the background and context for the chosen topic of interest, a list of the necessary materials and reagents needed for each topic, as well as the step-by-step laboratory protocols and methodologies for the successful and reproducible execution of each topic. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Inflammation and Cancer: Methods and Protocols aims to provide information to students and researchers at all levels of experience for further study into this vital field.

• COPD
• arthritis
• inflammasome
• cytokines
• malignancies

“Wide range of scientists exploring not only cancer biologic principles but as well those from immunology and inflammation fields may find this book a useful source of inspiring state-of-the-art molecular and cellular biological methodologies, useful techniques, research models, and even better – detailed protocols supported with pictures of critical steps in procedures, concrete scoring parameters used, along with tables summarizing important information, representative data, and calculations.” (Andrea Babelova, Neoplasma, Vol. 65 (4), 2018)

• Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical Research, Clayton, Australia

  Brendan J. Jenkins

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