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Glaucoma

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Tatjana C. Jakobs

    1. Department of Ophthalmology, Massachusetts Eye and Ear Infirmary/Schepens Eye, Research Institute, Harvard Medical School, Boston, USA

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  • Includes cutting-edge methods and protocols for the study of glaucoma
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1695)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Use of Animal Models and Techniques in Glaucoma Research: Introduction

    • Harry A. Quigley
    Pages 1-10

  3. Hypertonic Saline Injection Model of Experimental Glaucoma in Rats

    • John C. Morrison, Elaine C. Johnson, William O. Cepurna
    Pages 11-21

  4. The Microbead Occlusion Model of Ocular Hypertension in Mice

    • David J. Calkins, Wendi S. Lambert, Cathryn R. Formichella, William M. McLaughlin, Rebecca M. Sappington
    Pages 23-39

  5. Ocular Hypertension/Glaucoma in Minipigs: Episcleral Veins Cauterization and Microbead Occlusion Methods

    • Elena Vecino, Haritz Urcola, Alejando Bayon, Sansar C. Sharma
    Pages 41-48

  6. Noninvasive Intraocular Pressure Measurement in Animals Models of Glaucoma

    • Yan Hu, John Danias
    Pages 49-61

  7. High-Throughput Binocular Pattern Electroretinograms in the Mouse

    • Tsung-Han Chou, Jonathon Toft-Nielsen, Vittorio Porciatti
    Pages 63-68

  8. Visual Evoked Potentials in Glaucoma and Alzheimer’s Disease

    • Elisa Cerri, Carlotta Fabiani, Chiara Criscuolo, Luciano Domenici
    Pages 69-80

  9. Investigation of the Functional Retinal Output Using Microelectrode Arrays

    • Günther Zeck
    Pages 81-88

  10. Quantitative Proteomic Analysis of Human Aqueous Humor Using iTRAQ 4plex Labeling

    • Anna Trzeciecka, Padmanabhan Pattabiraman, Maria Carmen Piqueras, Carol Toris, Sanjoy K. Bhattacharya
    Pages 89-95

  11. Shotgun Sphingolipid Analysis of Human Aqueous Humor

    • Anna Trzeciecka, Ulises Arbelo, Arturo Barron, Genea Edwards, Sruthi Sampathkumar, Carol Toris et al.
    Pages 97-107

  12. Assessment of Aqueous Humor Dynamics in the Rodent by Constant Flow Infusion

    • J. Cameron Millar, Tien N. Phan, Iok-Hou Pang
    Pages 109-120

  13. Methods for Analyzing Endoplasmic Reticulum Stress in the Trabecular Meshwork of Glaucoma Models

    • Prabhavathi Maddineni, Ramesh B. Kasetti, Gulab S. Zode
    Pages 121-134

  14. Quantification of Scleral Biomechanics and Collagen Fiber Alignment

    • Ian C. Campbell, Joseph M. Sherwood, Darryl R. Overby, Bailey G. Hannon, A. Thomas Read, Julia Raykin et al.
    Pages 135-159

  15. Biolistic Labeling of Retinal Ganglion Cells

    • Luca Della Santina, Yvonne Ou
    Pages 161-170

  16. Anterograde Tract Tracing for Assaying Axonopathy and Transport Deficits in Glaucoma

    • Samuel D. Crish, Brett R. Schofield
    Pages 171-185

  17. In Vitro and In Vivo Methods for Studying Retinal Ganglion Cell Survival and Optic Nerve Regeneration

    • Yuqin Yin, Larry I. Benowitz
    Pages 187-205

  18. 3D Histomorphometric Reconstruction and Quantification of the Optic Nerve Head Connective Tissues

    • Hongli Yang, Juan Reynaud, Howard Lockwood, Galen Williams, Christy Hardin, Luke Reyes et al.
    Pages 207-267

  19. Visualizing Astrocytes of the Optic Nerve

    • Daniel Sun
    Pages 269-286

  20. Investigation of MicroRNA Expression in Experimental Glaucoma

    • Hari Jayaram, Diana C. Lozano, Elaine C. Johnson, John C. Morrison
    Pages 287-297
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Keywords

About this book

This detailed volume introduces the reader to current methods in glaucoma research, in particular those that make use of animal disease models. Given that the retinal ganglion cells damaged and destroyed by glaucoma do not regenerate after lowering the intraocular pressure, new therapeutic approaches that protect ganglion cells directly is a vital research goal that this volume’s contents aim to aid scientists in developing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Glaucoma: Methods and Protocols serves as a useful guide for both ophthalmologists in clinical practice and researchers in the field.

Editors and Affiliations

  • Department of Ophthalmology, Massachusetts Eye and Ear Infirmary/Schepens Eye, Research Institute, Harvard Medical School, Boston, USA

    Tatjana C. Jakobs

Bibliographic Information

  • Book Title: Glaucoma

  • Book Subtitle: Methods and Protocols

  • Editors: Tatjana C. Jakobs

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7407-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2018

  • Hardcover ISBN: 978-1-4939-7406-1Published: 01 December 2017

  • Softcover ISBN: 978-1-4939-8476-3Published: 30 August 2018

  • eBook ISBN: 978-1-4939-7407-8Published: 29 November 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 337

  • Number of Illustrations: 16 b/w illustrations, 77 illustrations in colour

  • Topics: Molecular Medicine

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