Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1678)
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Table of contents (20 protocols)
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Front Matter
About this book
This fourth edition volume expands on the previous editions by presenting readers with new and emerging methodologies in cytometry. The chapters in this book cover various topics such as quantifying surface and intracellular protein biomarkers, intracellular cytokine staining, apoptosis analysis, cell cycle analysis, tracking cell proliferation, and monitoring protein-protein interactions using FRET. Some developing methodologies that explored are mass cytometry, vesicle flow cytometry, time-resolved flow cytometry, and real-time label-free deformability cytometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Flow Cytometry Protocols, Fourth Edition  researchers and scientists who are interested in continuing or expanding their knowledge of flow cytometry.
Editors and Affiliations
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Flow Cytometry Section, Research Technologies Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, USA
Teresa S. Hawley
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Department of Anatomy and Regenerative Biology, School of Medicine and Health Sciences, George Washington University, Washington, USA
Robert G. Hawley
Bibliographic Information
Book Title: Flow Cytometry Protocols
Editors: Teresa S. Hawley, Robert G. Hawley
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-7346-0
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media LLC 2018
Hardcover ISBN: 978-1-4939-7344-6Published: 26 October 2017
Softcover ISBN: 978-1-4939-8459-6Published: 23 August 2018
eBook ISBN: 978-1-4939-7346-0Published: 24 October 2017
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 4
Number of Pages: XIV, 492
Number of Illustrations: 27 b/w illustrations, 80 illustrations in colour
Topics: Biotechnology