Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1681)
Buy it now
Buying options
Tax calculation will be finalised at checkout
Other ways to access
This is a preview of subscription content, log in via an institution to check for access.
Table of contents (22 protocols)
-
Front Matter
-
Key Basics of Phage Biology
-
Front Matter
-
-
Sequencing Analysis of Bacteriophages
-
Front Matter
-
-
Phage-Related Bioinformatics Tools
-
Front Matter
-
About this book
This book expands on the previous volumes with new chapters exploring emerging themes and methodologies in bacterial virus research. The chapters in this book are divided into 4 parts and cover topics such as: iron chloride flocculation of bacteriophages from seawater; encapsulation of Listeria phage A511 by alginate; examining genome termini of bacteriophage through high-throughput sequencing; genome sequencing of dsDNA-containing bacteriophages directly from a single plaque; characterizing bacteriophages by biology, taxonomy, and genome analysis; phage genome annotation using the RAST pipeline; and the use of RP4::mini-Mu for gene transfer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting edge and authoritative, Bacteriophages: Methods and Protocols, Volume IIIÂ is a valuable resource for both established and novice phage scientists.
Editors and Affiliations
-
Department Infection, Immunity & Inflammation, University of Leicester School of Medicine, Leicester, United Kingdom
Martha R.J. Clokie
-
University of Guelph, Guelph, Canada
Andrew M. Kropinski
-
KU Leuven, Laboratory of Gene Technology, Leuven, Belgium
Rob Lavigne
Bibliographic Information
Book Title: Bacteriophages
Book Subtitle: Methods and Protocols, Volume 3
Editors: Martha R.J. Clokie, Andrew M. Kropinski, Rob Lavigne
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-7343-9
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media LLC 2018
Hardcover ISBN: 978-1-4939-7341-5Published: 15 November 2017
Softcover ISBN: 978-1-4939-8458-9Published: 23 August 2018
eBook ISBN: 978-1-4939-7343-9Published: 10 November 2017
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 316
Number of Illustrations: 25 b/w illustrations, 36 illustrations in colour
Topics: Microbiology