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Wheat Biotechnology

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Prem L. Bhalla

    1. Plant Molecular Biology and Biotechnology Laboratory, School of Agriculture and Food, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Australia

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  2. Mohan B. Singh

    1. Plant Molecular Biology and Biotechnology Laboratory, School of Agriculture and Food, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Australia

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  • Includes cutting-edge techniques for the study of biotechnological approaches to wheat production
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1679)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
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  2. Background and Overview

    1. Front Matter

      Pages 1-1
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    2. Enabling Molecular Technologies for Trait Improvement in Wheat

      • Prem L. Bhalla, Akanksha Sharma, Mohan B. Singh
      Pages 3-24

    3. What Will Be the Benefits of Biotech Wheat for European Agriculture?

      • Agnès E. Ricroch
      Pages 25-35

    4. Overview of the Wheat Genetic Transformation and Breeding Status in China

      • Jiapeng Han, Xiaofen Yu, Junli Chang, Guangxiao Yang, Guangyuan He
      Pages 37-60

    5. Wheat Improvement in India: Present and Future

      • Sonia Goel, Kalpana Singh, N. K. Singh
      Pages 61-82

    6. Overview of Methods for Assessing Salinity and Drought Tolerance of Transgenic Wheat Lines

      • Rohit Joshi, Khalid Anwar, Priyanka Das, Sneh L. Singla-Pareek, Ashwani Pareek
      Pages 83-95

    7. Allergenicity Assessment of Transgenic Wheat Lines In Silico

      • Ankita Mishra, Naveen Arora
      Pages 97-111

    8. Agribusiness Perspectives on Transgenic Wheat

      • Bill Malcolm
      Pages 113-126

  3. Protocols

    1. Front Matter

      Pages 127-127
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    2. Agrobacterium-Mediated Transformation of Wheat Using Immature Embryos

      • Goetz Hensel, Cornelia Marthe, Jochen Kumlehn
      Pages 129-139

    3. Biolistic Transformation of Wheat

      • Caroline Tassy, Pierre Barret
      Pages 141-152

    4. Wheat Genetic Transformation Using Mature Embryos as Explants

      • Harsh Chauhan, Paramjit Khurana
      Pages 153-167

    5. Targeted Mutagenesis in Hexaploid Bread Wheat Using the TALEN and CRISPR/Cas Systems

      • Yanpeng Wang, Yuan Zong, Caixia Gao
      Pages 169-185

    6. Design and Assembly of CRISPR/Cas9 Reagents for Gene Knockout, Targeted Insertion, and Replacement in Wheat

      • Tomáš ÄŒermák, Shaun J. Curtin
      Pages 187-212

    7. Doubled Haploid Transgenic Wheat Lines by Microspore Transformation

      • Sachin Rustgi, Nii O. Ankrah, Rhoda A. T. Brew-Appiah, Yue Sun, Weiguo Liu, Diter von Wettstein
      Pages 213-234

    8. Doubled Haploid Laboratory Protocol for Wheat Using Wheat–Maize Wide Hybridization

      • Meenakshi Santra, Hong Wang, Scott Seifert, Scott Haley
      Pages 235-249

    9. Real-Time PCR for the Detection of Precise Transgene Copy Number in Wheat

      • Angelica Giancaspro, Agata Gadaleta, Antonio Blanco
      Pages 251-257

    10. Endogenous Reference Genes and Their Quantitative Real-Time PCR Assays for Genetically Modified Bread Wheat (Triticum aestivum L.) Detection

      • Litao Yang, Sheng Quan, Dabing Zhang
      Pages 259-268

    11. Phenotypic Characterization of Transgenic Wheat Lines Against Fungal Pathogens Puccinia triticina and Fusarium graminearum

      • Jagdeep Kaur, Dilip Shah, John Fellers
      Pages 269-276
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Keywords

About this book

This detailed volume provides background on recent new technology developments highlighting the potential of the genomic era in wheat breeding with invaluable instruction on the methodology, which is complemented by overview chapters on the status of new technology application in major wheat production countries. The topics, addressed by internationally renowned scientists active in the field, cover methods underpinning the latest developments in the field of wheat biotechnology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Wheat Biotechnology: Methods and Protocols serves as a vital resource for scientists working to breed future high-yielding wheat varieties to sustaina growing population in an increasingly unpredictable world.

Editors and Affiliations

  • Plant Molecular Biology and Biotechnology Laboratory, School of Agriculture and Food, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Australia

    Prem L. Bhalla, Mohan B. Singh

Bibliographic Information

  • Book Title: Wheat Biotechnology

  • Book Subtitle: Methods and Protocols

  • Editors: Prem L. Bhalla, Mohan B. Singh

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7337-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7335-4Published: 15 September 2017

  • Softcover ISBN: 978-1-4939-8456-5Published: 18 May 2018

  • eBook ISBN: 978-1-4939-7337-8Published: 14 September 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 309

  • Number of Illustrations: 3 b/w illustrations, 50 illustrations in colour

  • Topics: Plant Sciences

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