Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1677)
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Table of contents (17 protocols)
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NMDAR Subunits Expression and Mutations
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Studying Structure–Function Relations in NMDARs
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Analysis of NMDAR Function/Activity
Keywords
About this book
This volume explores the latest techniques used by researchers to help them better understand the NMDAR structure-function relations, principles, and rules that govern how NMDARs operate in brain processing. The volume includes a detailed introductory chapter describing the field and is divided into three parts. Topics covered in the volume include: quantification of NMDAR subunit genes expression by qRT-PCR; detection of NMDARs antibodies in encephalitis; recombinant channels in host cells using a fast agonist application system; GluNs detection and functions in microglial cells; and NMDARs as voltage sensors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, NMDA Receptors: Methods and Protocols is a valuable resource that discusses the diversity of the currently-used methods, the importance of NMDARs and their complexity, and the progress that has already been made. This book will be of interest to scientists, clinicians, and industry professionals working in this field.
Editors and Affiliations
Bibliographic Information
Book Title: NMDA Receptors
Book Subtitle: Methods and Protocols
Editors: Nail Burnashev, Pierre Szepetowski
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-7321-7
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media LLC 2017
Hardcover ISBN: 978-1-4939-7320-0Published: 07 October 2017
Softcover ISBN: 978-1-4939-8452-7Published: 14 August 2018
eBook ISBN: 978-1-4939-7321-7Published: 06 October 2017
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIII, 314
Number of Illustrations: 31 b/w illustrations, 46 illustrations in colour
Topics: Neurosciences