Plant Germline Development

1. Front Matter

   Pages i-xiii

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2. Plant Germline Development: From Investigation to Application

   1. Front Matter

      Pages 1-1

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   2. An Introduction to Male Germline Development

      • Hannes Vogler, Ueli Grossniklaus, Afif Hedhly

      Pages 3-15

   3. Apomixis: Engineering the Ability to Harness Hybrid Vigor in Crop Plants

      • Joann A. Conner, Peggy Ozias-Akins

      Pages 17-34

3. Clearing, Imaging Techniques, and Immunolocalization to Study Cell Lineage, Cell Type Specificity, Cytoskeleton, Cell Cycle, Signaling, and Hormones

   1. Front Matter

      Pages 35-35

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   2. 3D Imaging of Whole-Mount Ovules at Cellular Resolution to Study Female Germline Development in Rice

      • Ethel Mendocilla-Sato, Wenjing She, Célia Baroux

      Pages 37-45

   3. Live-Cell Imaging of F-Actin Dynamics During Fertilization in Arabidopsis thaliana

      • Daichi Susaki, Daisuke Maruyama, Ramesh Yelagandula, Frederic Berger, Tomokazu Kawashima

      Pages 47-54

   4. Development and Observation of Mature Megagametophyte Cell-Specific Fluorescent Markers

      • Mark A. Chamberlin, Shai J. Lawit

      Pages 55-65

   5. Analysis of Fluorescent Reporter Activity in the Male Germline During Pollen Development by Confocal Microscopy

      • David Twell, Lynette Brownfield

      Pages 67-75

   6. In Vivo Ploidy Determination of Arabidopsis thaliana Male and Female Gametophytes

      • Burcu Nur Keçeli, Nico De Storme, Danny Geelen

      Pages 77-85

   7. Staining and Clearing of Arabidopsis Reproductive Tissue for Imaging of Fluorescent Proteins

      • Daniel Slane, Patrick Bürgel, Martin Bayer

      Pages 87-94

   8. Live-Cell Imaging of Auxin and Cytokinin Signaling in Maize Female Gametophytes

      • Antony M. Chettoor, Matthew M. S. Evans

      Pages 95-101

   9. Imaging Ca2+ Dynamics in Wild-Type and NADPH Oxidase-Deficient Mutant Pollen Tubes with Yellow Cameleon and Confocal Laser Scanning Microscopy

      • Christina Maria Franck, Jens Westermann, Aurélien Boisson-Dernier

      Pages 103-116

   10. Immunolocalization of AGPs and Pectins in Quercus suber Gametophytic Structures

       • Mário Luís da Costa, Ana Lúcia Lopes, Maria Isabel Amorim, Sílvia Coimbra

       Pages 117-137

4. Chromosome Spreading, Whole Mount In Situ, and Transmission Electron Microscopy (TEM)

   1. Front Matter

      Pages 139-139

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   2. Optimization of Cell Spreading and Image Quality for the Study of Chromosomes in Plant Tissues

      • Laksana Kantama, Erik Wijnker, Hans de Jong

      Pages 141-158

   3. Whole Mount RNA-FISH on Ovules and Developing Seeds

      • Andrea Bleckmann, Thomas Dresselhaus

      Pages 159-171

   4. Analysis of Peroxisome Biogenesis in Pollen by Confocal Microscopy and Transmission Electron Microscopy

      • Peng-Fei Jia, Hong-Ju Li, Wei-Cai Yang

      Pages 173-180

   5. Transmission Electron Microscopy (TEM) to Study Histology of Pollen and Pollen Tubes

      • Peng-Fei Jia, Hong-Ju Li, Wei-Cai Yang

      Pages 181-189

5. Isolation of Specific Cells of the Male and Female Germlines

   1. Front Matter

      Pages 191-191

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This detailed volume explores common and numerous specialized methods to study various aspects of plant germline development and targeted manipulation, including imaging and hybridization techniques to study cell-type specification, cell lineage, signaling and hormones, cell cycle, and the cytoskeleton. In addition, cell-type specific methods for targeted ablation or isolation are provided, protocols to apply “omics” technologies and to perform bioinformatics data analysis, as well as methods relevant for aspects of biotechnology or plant breeding. This includes protocols that are relevant for the targeted manipulation of pathways, for crop plant transformation, or for conditional induction of phenotypes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Plant Germline Development: Methods and Protocols serves as a comprehensive guide not only to studying basic questions related to different aspects of plant reproductive development but also for state of the art methods, in addition to being a source of inspiration for new approaches and research questions in many laboratories.

• Plant reproduction
• Cell lineage
• Imaging techniques
• Crop plant transformation
• Developmental genetics
• Crop improvement

• Centre for Organismal Studies, University of Heidelberg, Heidelberg, Germany

  Anja Schmidt

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