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Skeletal Muscle Development

  • Book
  • © 2017

Overview

Editors:
  1. James G. Ryall

    1. Department of Physiology, The University of Melbourne, Parkville, Australia

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  • Includes cutting-edge methods and protocols for the study of skeletal muscle cell development
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1668)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Skeletal Muscle Gene Expression

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Bisulfite Sequencing for DNA Methylation Analysis of Primary Muscle Stem Cells

      • Kohei Miyata, Masashi Naito, Tomoko Miyata, Sho Mokuda, Hiroshi Asahara
      Pages 3-13

    3. Whole Genome Chromatin IP-Sequencing (ChIP-Seq) in Skeletal Muscle Cells

      • Karl Kamhei So, Xianlu Laura Peng, Hao Sun, Huating Wang
      Pages 15-25

    4. Analysis of RNA Expression in Adult Zebrafish Skeletal Muscle

      • Tamar E. Sztal, Peter D. Currie, Robert J. Bryson-Richardson
      Pages 27-35

  3. Assays for Proliferating Skeletal Muscle Cells

    1. Front Matter

      Pages 37-37
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    2. Targeted Lipidomic Analysis of Myoblasts by GC-MS and LC-MS/MS

      • Jordan Blondelle, Jean-Paul Pais de Barros, Fanny Pilot-Storck, Laurent Tiret
      Pages 39-60

    3. Measuring Mitochondrial Substrate Utilization in Skeletal Muscle Stem Cells

      • C. Hai Ly, James G. Ryall
      Pages 61-73

    4. Microcontact-Printed Hydrogel Microwell Arrays for Clonal Muscle Stem Cell Cultures

      • Victor M. Aguilar, Benjamin D. Cosgrove
      Pages 75-92

    5. Isolation, Culture, and Differentiation of Fibro/Adipogenic Progenitors (FAPs) from Skeletal Muscle

      • Robert N. Judson, Marcela Low, Christine Eisner, Fabio M. Rossi
      Pages 93-103

    6. Human Satellite Cell Isolation and Xenotransplantation

      • Steven M. Garcia, Stanley Tamaki, Xiaoti Xu, Jason H. Pomerantz
      Pages 105-123

  4. Assessment of Myogenic Differentiation

    1. Front Matter

      Pages 125-125
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    2. Application of Split-GFP Reassembly Assay to Study Myogenesis and Myofusion In Vitro

      • Manami Kodaka, Xiaoyin Xu, Zeyu Yang, Junichi Maruyama, Yutaka Hata
      Pages 127-134

    3. Myogenic Maturation by Optical-Training in Cultured Skeletal Muscle Cells

      • Toshifumi Asano, Toru Ishizuka, Hiromu Yawo
      Pages 135-145

    4. Fabrication of Micromolded Gelatin Hydrogels for Long-Term Culture of Aligned Skeletal Myotubes

      • Gio C. Suh, Archana Bettadapur, Jeffrey W. Santoso, Megan L. McCain
      Pages 147-163

  5. Assessing Skeletal Muscle Development In Vivo

    1. Front Matter

      Pages 165-165
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    2. Quantification of Embryonic Myofiber Development by Immunofluorescence

      • Harika Nagandla, M. David Stewart
      Pages 167-176

    3. How to Wire the Diaphragm: Wholemount Staining Methods to Analyze Mammalian Respiratory Innervation

      • Maximilian Michael Saller, Paolo Alberton, Andrea B. Huber, Rosa-Eva Huettl
      Pages 177-192

  6. Muscle Repair Assays

    1. Front Matter

      Pages 193-193
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    2. Membrane Repair Assay for Human Skeletal Muscle Cells

      • Romain Carmeille, Coralie Croissant, Flora Bouvet, Anthony Bouter
      Pages 195-207
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Keywords

About this book

This detailed volume presents a wide range of techniques that go beyond the standard assays typically used to assess Myogenesis. The content included addresses assays to analyze skeletal muscle gene expression, proliferating muscle cells, the process of myoblast fusion, muscle development (in vivo), as well as muscle repair. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Skeletal Muscle Development serves as an ideal guide to the study of this highly complex yet carefully regulated process.>

Editors and Affiliations

  • Department of Physiology, The University of Melbourne, Parkville, Australia

    James G. Ryall

Bibliographic Information

  • Book Title: Skeletal Muscle Development

  • Editors: James G. Ryall

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7283-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7282-1Published: 25 August 2017

  • Softcover ISBN: 978-1-4939-8441-1Published: 12 May 2018

  • eBook ISBN: 978-1-4939-7283-8Published: 25 August 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 226

  • Number of Illustrations: 7 b/w illustrations, 46 illustrations in colour

  • Topics: Cell Biology

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